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Genome-wide identification and expression analysis of the C2H2-zinc finger transcription factor gene family and screening of candidate genes involved in floral development in Coptis teeta Wall. (Ranunculaceae)
Duan, Shao-Feng Zhao, Yan Yu, Ji-Chen Xiang, Gui-Sheng Xiao, Lin Cui, Rui Hu, Qian-Qian Lu, Ying-Chun Liang, Yan-Li
Duan, Shao-Feng
Zhao, Yan
Yu, Ji-Chen
Xiang, Gui-Sheng
Xiao, Lin
Cui, Rui
Hu, Qian-Qian
Lu, Ying-Chun
Liang, Yan-Li
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2024-01-22
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Abstract
Background: C2H2-zinc finger transcription factors comprise one of the largest and most diverse gene superfamilies and are involved in the transcriptional regulation of flowering. Although a large number of C2H2 zinc-finger proteins (C2H2-ZFPs) have been well characterized in a number of model plant species, little is known about their expression and function in Coptis teeta. C. teeta displays two floral phenotypes (herkogamy phenotypes). It has been proposed that the C2H2-zinc finger transcription factor family may play a crucial role in the formation of floral development and herkogamy observed in C. teeta. As such, we performed a genome-wide analysis of the C2H2-ZFP gene family in C. teeta. Results: The complexity and diversity of C. teeta C2H2 zinc finger proteins were established by evaluation of their physicochemical properties, phylogenetic relationships, exon-intron structure, and conserved motifs. Chromosome localization showed that 95 members of the C2H2 zinc-finger genes were unevenly distributed across the nine chromosomes of C. teeta, and that these genes were replicated in tandem and segmentally and had undergone purifying selection. Analysis of cis-acting regulatory elements revealed a possible involvement of C2H2 zinc-finger proteins in the regulation of phytohormones. Transcriptome data was then used to compare the expression levels of these genes during the growth and development of the two floral phenotypes (F-type and M-type). These data demonstrate that in groups A and B, the expression levels of 23 genes were higher in F-type flowers, while 15 genes showed higher expressions in M-type flowers. qRT-PCR analysis further revealed that the relative expression was highly consistent with the transcriptome data. Conclusion: These data provide a solid basis for further in-depth studies of the C2H2 zinc finger transcription factor gene family in this species and provide preliminary information on which to base further research into the role of the C2H2 ZFPs gene family in floral development in C. teeta.
Citation
Duan, S-F., Zhao, Y., Yu, J-C., Xiang, G-S., Xiao, L., Cui, R., Hu, Q-Q., Baldwin, T.C., Lu, Y-C. and Liang, Y-L. (2024) Genome-wide identification and expression analysis of the C2H2-zinc finger transcription factor gene family and screening of candidate genes involved in floral development in Coptis teeta Wall. (Ranunculaceae). Frontiers in Genetics, 15:1349673. doi:10.3389/fgene.2024.1349673
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Journal article
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en
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© 2023 The authors. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.3389/fgene.2024.1349673
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1664-8021
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1664-8021
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This research was supported by the National Natural Science Foundation of China (project grant no: 31971543), Major Special Projects of Yunnan Province (Biomedicine) (project number: 202102AA310045), Yunnan Provincial Science and Technology Department Agriculture Joint Special Project (projectnumber:202101BD070001-008).