CEL1: a novel cellulose binding protein secreted by Agaricus bisporus during growth on crystalline cellulose.
Abstract
The cel1 gene of Agaricus bisporus encodes a protein (CEL1) that has an architecture resembling the multi-domain fungal cellulases, although the sequence of its putative catalytic core is not matched by any other in the protein and nucleic acid data bases. The N-terminal half of the putative catalytic domain of CEL1 was expressed in Escherichia coli as a fusion protein with glutathione-S-transferase. The fusion protein was used to raise a CEL1-specific antibody. CEL1 was detected as an extracellular 49.8 kDa protein in A. bisporus cellulose-grown cultures, where it bound strongly to cellulose. CEL1 was neither an endoglucanase, a cellobiohydrolase able to hydrolyze fluorogenic cellobiosides, a beta-glucosidase, a xylanase, nor a cellobiose: quinone oxidoreductase. CEL1 was present in some fractions of culture fluid separated by electrophoresis which released soluble sugars from crystalline cellulose.Citation
FEMS Microbiology Letters, 116(3): 293-299Publisher
Blackwell PublishingPubMed ID
8181702Additional Links
http://www3.interscience.wiley.com/journal/119276698/abstractType
Journal articleLanguage
enDescription
Metadata onlyISSN
0378-1097ae974a485f413a2113503eed53cd6c53
10.1111/j.1574-6968.1994.tb06718.x
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