Genetic import and phenotype specific alleles associated with hyper-invasion in Campylobacter jejuni

2.50
Hdl Handle:
http://hdl.handle.net/2436/621195
Title:
Genetic import and phenotype specific alleles associated with hyper-invasion in Campylobacter jejuni
Authors:
Baig, Abiyad; McNally, Alan; Dunn, Steven; Paszkiewicz, Konrad H.; Corander, Jukka; Manning, Georgina ( 0000-0003-2198-0880 )
Abstract:
Background Campylobacter jejuni is a major zoonotic pathogen, causing gastroenteritis in humans. Invasion is an important pathogenesis trait by which C. jejuni causes disease. Here we report the genomic analysis of 134 strains to identify traits unique to hyperinvasive isolates. Methods A total of 134 C. jejuni genomes were used to create a phylogenetic tree to position the hyperinvasive strains. Comparative genomics lead to the identification of mosaic capsule regions. A pan genome approach led to the discovery of unique loci, or loci with unique alleles, to the hyperinvasive strains. Results Phylogenetic analysis showed that the hyper-invasive phenotype is a generalist trait. Despite the fact that hyperinvasive strains are only distantly related based on the whole genome phylogeny, they all possess genes within the capsule region with high identity to capsule genes from C. jejuni subsp. doylei and C. lari. In addition there were genes unique to the hyper-invasive strains with identity to non-C. jejuni genes, as well as allelic variants of mainly pathogenesis related genes already known in the other C. jejuni. In particular, the sequence of flagella genes, flgD-E and flgL were highly conserved amongst the hyper-invasive strains and divergent from sequences in other C. jejuni. A novel cytolethal distending toxin (cdt) operon was also identified as present in all hyper-invasive strains in addition to the classic cdt operon present in other C. jejuni. Conclusions Overall, the hyper-invasive phenotype is strongly linked to the presence of orthologous genes from other Campylobacter species in their genomes, notably within the capsule region, in addition to the observed association with unique allelic variants in flagellar genes and the secondary cdt operon which is unlikely under random sharing of accessory alleles in separate lineages.
Citation:
Genetic import and phenotype specific alleles associated with hyper-invasion in Campylobacter jejuni 2015, 16 (1) BMC Genomics
Publisher:
BioMed Central
Journal:
BMC Genomics
Issue Date:
24-Oct-2015
URI:
http://hdl.handle.net/2436/621195
DOI:
10.1186/s12864-015-2087-y
Additional Links:
http://www.biomedcentral.com/1471-2164/16/852
Type:
Article
Language:
en
ISSN:
1471-2164
Sponsors:
Nottingham Trent University
Appears in Collections:
FSE

Full metadata record

DC FieldValue Language
dc.contributor.authorBaig, Abiyaden
dc.contributor.authorMcNally, Alanen
dc.contributor.authorDunn, Stevenen
dc.contributor.authorPaszkiewicz, Konrad H.en
dc.contributor.authorCorander, Jukkaen
dc.contributor.authorManning, Georginaen
dc.date.accessioned2018-03-21T13:07:35Z-
dc.date.available2018-03-21T13:07:35Z-
dc.date.issued2015-10-24-
dc.identifier.citationGenetic import and phenotype specific alleles associated with hyper-invasion in Campylobacter jejuni 2015, 16 (1) BMC Genomicsen
dc.identifier.issn1471-2164-
dc.identifier.doi10.1186/s12864-015-2087-y-
dc.identifier.urihttp://hdl.handle.net/2436/621195-
dc.description.abstractBackground Campylobacter jejuni is a major zoonotic pathogen, causing gastroenteritis in humans. Invasion is an important pathogenesis trait by which C. jejuni causes disease. Here we report the genomic analysis of 134 strains to identify traits unique to hyperinvasive isolates. Methods A total of 134 C. jejuni genomes were used to create a phylogenetic tree to position the hyperinvasive strains. Comparative genomics lead to the identification of mosaic capsule regions. A pan genome approach led to the discovery of unique loci, or loci with unique alleles, to the hyperinvasive strains. Results Phylogenetic analysis showed that the hyper-invasive phenotype is a generalist trait. Despite the fact that hyperinvasive strains are only distantly related based on the whole genome phylogeny, they all possess genes within the capsule region with high identity to capsule genes from C. jejuni subsp. doylei and C. lari. In addition there were genes unique to the hyper-invasive strains with identity to non-C. jejuni genes, as well as allelic variants of mainly pathogenesis related genes already known in the other C. jejuni. In particular, the sequence of flagella genes, flgD-E and flgL were highly conserved amongst the hyper-invasive strains and divergent from sequences in other C. jejuni. A novel cytolethal distending toxin (cdt) operon was also identified as present in all hyper-invasive strains in addition to the classic cdt operon present in other C. jejuni. Conclusions Overall, the hyper-invasive phenotype is strongly linked to the presence of orthologous genes from other Campylobacter species in their genomes, notably within the capsule region, in addition to the observed association with unique allelic variants in flagellar genes and the secondary cdt operon which is unlikely under random sharing of accessory alleles in separate lineages.en
dc.description.sponsorshipNottingham Trent Universityen
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2164/16/852en
dc.rightsArchived with thanks to BMC Genomicsen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectGenetic importen
dc.subjectCampylobacteren
dc.subjectinvasionen
dc.titleGenetic import and phenotype specific alleles associated with hyper-invasion in Campylobacter jejunien
dc.typeArticleen
dc.identifier.journalBMC Genomicsen
This item is licensed under a Creative Commons License
Creative Commons
All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.