Modulation of mitochondrial activity in HaCaT keratinocytes by the cell penetrating peptide Z-Gly-RGD(DPhe)-mitoparan

2.50
Hdl Handle:
http://hdl.handle.net/2436/621065
Title:
Modulation of mitochondrial activity in HaCaT keratinocytes by the cell penetrating peptide Z-Gly-RGD(DPhe)-mitoparan
Authors:
Richardson, Adam; Muir, Lewis; Mousdell, Sasha; Sexton, Darren; Jones, Sarah; Howl, John; Ross, Kehinde ( 0000-0003-0252-1152 )
Abstract:
Objective Biologically active cell penetrating peptides (CPPs) are an emerging class of therapeutic agent. The wasp venom peptide mastoparan is an established CPP that modulates mitochondrial activity and triggers caspase-dependent apoptosis in cancer cells, as does the mastoparan analogue mitoparan (mitP). Mitochondrial depolarisation and activation of the caspase cascade also underpins the action of dithranol, a topical agent for treatment of psoriasis. The effects of a potent mitP analogue on mitochondrial activity were therefore examined to assess its potential as a novel approach for targeting mitochondria for the treatment of psoriasis. Results In HaCaT keratinocytes treated with the mitP analogue Z-Gly-RGD(DPhe)-mitP for 24 h, a dose-dependent loss of mitochondrial activity was observed using the methyl-thiazolyl-tetrazolium (MTT) assay. At 10 μmol L−1, MTT activity was less than 30% that observed in untreated cells. Staining with the cationic dye JC-1 suggested that Z-Gly-RGD(DPhe)-mitP also dissipated the mitochondrial membrane potential, with a threefold increase in mitochondrial depolarisation levels. However, caspase activity appeared to be reduced by 24 h exposure to Z-Gly-RGD(DPhe)-mitP treatment. Furthermore, Z-Gly-RGD(DPhe)-mitP treatment had little effect on overall cell viability. Our findings suggest Z-Gly-RGD(DPhe)-mitP promotes the loss of mitochondrial activity but does not appear to evoke apoptosis in HaCaT keratinocytes.
Citation:
Modulation of mitochondrial activity in HaCaT keratinocytes by the cell penetrating peptide Z-Gly-RGD(DPhe)-mitoparan 2018, 11 (1) BMC Research Notes
Publisher:
BioMed Central
Journal:
BMC Research Notes
Issue Date:
30-Jan-2018
URI:
http://hdl.handle.net/2436/621065
DOI:
10.1186/s13104-018-3192-1
Additional Links:
https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3192-1
Type:
Article
Language:
en
ISSN:
1756-0500
Appears in Collections:
FSE

Full metadata record

DC FieldValue Language
dc.contributor.authorRichardson, Adamen
dc.contributor.authorMuir, Lewisen
dc.contributor.authorMousdell, Sashaen
dc.contributor.authorSexton, Darrenen
dc.contributor.authorJones, Sarahen
dc.contributor.authorHowl, Johnen
dc.contributor.authorRoss, Kehindeen
dc.date.accessioned2018-02-01T11:51:23Z-
dc.date.available2018-02-01T11:51:23Z-
dc.date.issued2018-01-30-
dc.identifier.citationModulation of mitochondrial activity in HaCaT keratinocytes by the cell penetrating peptide Z-Gly-RGD(DPhe)-mitoparan 2018, 11 (1) BMC Research Notesen
dc.identifier.issn1756-0500en
dc.identifier.doi10.1186/s13104-018-3192-1-
dc.identifier.urihttp://hdl.handle.net/2436/621065-
dc.description.abstractObjective Biologically active cell penetrating peptides (CPPs) are an emerging class of therapeutic agent. The wasp venom peptide mastoparan is an established CPP that modulates mitochondrial activity and triggers caspase-dependent apoptosis in cancer cells, as does the mastoparan analogue mitoparan (mitP). Mitochondrial depolarisation and activation of the caspase cascade also underpins the action of dithranol, a topical agent for treatment of psoriasis. The effects of a potent mitP analogue on mitochondrial activity were therefore examined to assess its potential as a novel approach for targeting mitochondria for the treatment of psoriasis. Results In HaCaT keratinocytes treated with the mitP analogue Z-Gly-RGD(DPhe)-mitP for 24 h, a dose-dependent loss of mitochondrial activity was observed using the methyl-thiazolyl-tetrazolium (MTT) assay. At 10 μmol L−1, MTT activity was less than 30% that observed in untreated cells. Staining with the cationic dye JC-1 suggested that Z-Gly-RGD(DPhe)-mitP also dissipated the mitochondrial membrane potential, with a threefold increase in mitochondrial depolarisation levels. However, caspase activity appeared to be reduced by 24 h exposure to Z-Gly-RGD(DPhe)-mitP treatment. Furthermore, Z-Gly-RGD(DPhe)-mitP treatment had little effect on overall cell viability. Our findings suggest Z-Gly-RGD(DPhe)-mitP promotes the loss of mitochondrial activity but does not appear to evoke apoptosis in HaCaT keratinocytes.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttps://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3192-1en
dc.rightsArchived with thanks to BMC Research Notesen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCell penetrating peptidesen
dc.subjectBioportidesen
dc.subjectKeratinocytesen
dc.subjectMitochondriaen
dc.titleModulation of mitochondrial activity in HaCaT keratinocytes by the cell penetrating peptide Z-Gly-RGD(DPhe)-mitoparanen
dc.typeArticleen
dc.identifier.journalBMC Research Notesen
dc.date.accepted2018-01-
rioxxterms.funderInternalen
rioxxterms.identifier.projectUoW010218SJen
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttps://creativecommons.org/CC BY-NC-ND 4.0en
rioxxterms.licenseref.startdate2018-02-01en
This item is licensed under a Creative Commons License
Creative Commons
All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.