Generation and characterization of monoclonal antibodies to the neural crest.
Authors
Shakil, T.Richardson, M. K.
Waldron, E.E.
Conde, Gillian
Wood, S.
Bland, Y.
Reynolds, Gary
Murray, Paul G.
Nelson, Paul N.
Issue Date
2001
Metadata
Show full item recordAbstract
The generation of monoclonal antibodies (MAbs) specific for quail neural crest may provide valuable tools for studying the differentiation of embryonic precursor cells. Unfortunately, relatively few antibodies are available because of the difficulty in obtaining sufficient cells for in vivo immunization strategies. We have overcome this problem by using intrasplenic immunization with formaldehyde-fixed cells harvested from neural crest cultures. In addition, booster injections of cultured whole-embryo cells were administered intraperitoneally. Following two fusions, a total of 18 hybridomas were generated with antibody reactivity to the cytoplasm of neural crest cells. Furthermore, 32 were reactive against both somite (a noncrest mesodermal control) and crest cultures, whilst 15 were not reactive. Out of those hybridomas reactive with neural crest, six designated 160D, 164D, OE, 12E, 120E and 124E were further characterized. Interestingly MAb supernatants OE, 12E, 120E, and 124E exhibited reactivity against some but not all neural crest cells suggesting that they might recognise subpopulations. Immunoglobulin isotyping of supernatants revealed that 4 (160D, 164D, OE, and 120E) were IgM and 2 (12E and 124E) were IgG(2b). On assessing their reactivity against human tissue sections, all six hybridoma supernatants cross-reacted with neuroendocrine cells within appendix, colon and rectum. These MAbs could provide novel reagents for the understanding of neural crest development.Citation
Hybridoma, 20(3): 199-203Journal
HybridomaPubMed ID
11461669Type
Journal articleLanguage
enISSN
0272-457Xae974a485f413a2113503eed53cd6c53
10.1089/027245701750293538
Scopus Count
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