Authors
Nelson, Paul N.Astley, S.J.
Roden, Denise A.
Waldron, E.E.
Baig, K.M.
Caforio, A.L.
Koutedakis, Yiannis
Perera, Shantha
Spry, C.
Issue Date
2005
Metadata
Show full item recordAbstract
The characterization of monoclonal antibodies (MAbs) with regard to reactivity and specificity is important for the successful application as a molecular probe and/or diagnostic reagent. Furthermore, it is recognized that some monoclonal reagents perform well in some assay systems but not others. In this study, the reactivity profiles of two anti-myosin MAbs (H1 and DH2, raised against human cardiac myosin) were evaluated in enzyme-linked immunosorbent assay (ELISA), slot-blotting, and immunocytochemistry. Both antibodies performed well in slot-blotting against myosin heavy chain preparations from cardiac and skeletal muscle and from non-human sources. In general, MAb H1 demonstrated strong to moderate reactivity in all assay systems, whilst MAb DH2 faired poorly in ELISA. MAb H1 also showed reactivity to synthetic peptides of myosin, one of which possessed a motif (ERRDA, single amino acid code) that was found in other human and nonhuman myosin protein sequences that could explain its cross-reactive profile. Intriguingly, this motif was found on viral and other pathogenic agents associated with myocarditis. Hence, it is speculated that this region could give some credence to the mechanism of molecular mimicry associated with some cardiac diseases. Overall, MAb H1 may serve as a useful probe of myosin structure.Citation
Hybridoma, 24(6): 314-318Journal
HybridomaPubMed ID
16332199Additional Links
https://www.liebertpub.com/doi/abs/10.1089/hyb.2005.24.314Type
Journal articleLanguage
enISSN
1554-0014ae974a485f413a2113503eed53cd6c53
10.1089/hyb.2005.24.314
Scopus Count
Collections
Related articles
- Production and characterization of monoclonal antibodies against vaginolysin: mapping of a region critical for its cytolytic activity.
- Authors: Zvirbliene A, Pleckaityte M, Lasickiene R, Kucinskaite-Kodze I, Zvirblis G
- Issue date: 2010 Aug 1
- Two monoclonal antibodies to precisely the same epitope of type II collagen select non-crossreactive phage clones by phage display: implications for autoimmunity and molecular mimicry.
- Authors: Xu Y, Ramsland PA, Davies JM, Scealy M, Nandakumar KS, Holmdahl R, Rowley MJ
- Issue date: 2004 Jun
- Murine anti-cytomegalovirus monoclonal antibodies with autoreactivity.
- Authors: Lawson CM, O'Donoghue HL, Farrell HE, Shellam GR, Reed WD
- Issue date: 1991 Mar
- Production of a human monoclonal IgM directed against human cardiac myosin in a hollow-fiber bioreactor for membrane anion exchange chromatography one-step purification.
- Authors: Jacobin MJ, Santarelli X, Laroche-Traineau J, Clofent-Sanchez G
- Issue date: 2004
- HLAMatchmaker-based analysis of human monoclonal antibody reactivity demonstrates the importance of an additional contact site for specific recognition of triplet-defined epitopes.
- Authors: Duquesnoy RJ, Mulder A, Askar M, Fernandez-Vina M, Claas FH
- Issue date: 2005 Jul