Mann, ERYou, JHorneffer-Van Der Sluis, VBernardo, DOmar Al-Hassi, HLandy, JPeake, STThomas, LVTee, CTLee, GHHart, ALYaqoob, PKnight, SC2020-07-172020-07-172013-07-18Mann, E. R., You, J., Horneffer-van der Sluis, V., Bernardo, D., Omar Al-Hassi, H., Landy, J., Peake, S. T., Thomas, L. V., Tee, C. T., Lee, G. H., Hart, A. L., Yaqoob, P. and Knight, S. C. (2013) Dysregulated circulating dendritic cell function in ulcerative colitis is partially restored by probiotic strain Lactobacillus casei Shirota. Mediators of inflammation, 2013. 573576.0962-935123970814 (pubmed)10.1155/2013/573576http://hdl.handle.net/2436/623363© 2013 The Authors. Published by Hindawi. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1155/2013/573576Background. Dendritic cells regulate immune responses to microbial products and play a key role in ulcerative colitis (UC) pathology. We determined the immunomodulatory effects of probiotic strain Lactobacillus casei Shirota (LcS) on human DC from healthy controls and active UC patients. Methods. Human blood DC from healthy controls (control-DC) and UC patients (UC-DC) were conditioned with heat-killed LcS and used to stimulate allogeneic T cells in a 5-day mixed leucocyte reaction. Results. UC-DC displayed a reduced stimulatory capacity for T cells (P<0.05) and enhanced expression of skin-homing markers CLA and CCR4 on stimulated T cells (P<0.05) that were negative for gut-homing marker β7. LcS treatment restored the stimulatory capacity of UC-DC, reflecting that of control-DC. LcS treatment conditioned control-DC to induce CLA on T cells in conjunction with β7, generating a multihoming profile, but had no effects on UC-DC. Finally, LcS treatment enhanced DC ability to induce TGFβ production by T cells in controls but not UC patients. Conclusions. We demonstrate a systemic, dysregulated DC function in UC that may account for the propensity of UC patients to develop cutaneous manifestations. LcS has multifunctional immunoregulatory activities depending on the inflammatory state; therapeutic effects reported in UC may be due to promotion of homeostasis. © 2013 Elizabeth R. Mann et al.application/pdfenLicence for published version: Creative Commons Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Dendritic CellsT-LymphocytesCells, CulturedHumansLactobacillus caseiColitis, UlcerativeInflammationTransforming Growth Factor betaCytokinesFlow CytometryLymphocyte ActivationCell ProliferationHomeostasisProbioticsJournal article1466-1861Mediators of Inflammation2020-06-30