Zhang, JingBellani, Marina A.James, Ryan C.Pokharel, DurgaZhang, YongqingReynolds, John J.McNee, GavinJackson, Andrew P.Stewart, Grant S.Seidman, Michael M.2025-04-032025-04-032020-08-07Zhang, J., Bellani, M.A., James, R.C. et al. (2020) DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain. Nature Communications 11, 3951. https://doi.org/10.1038/s41467-020-17449-12041-172332769987 (pubmed)10.1038/s41467-020-17449-1https://wlv.openrepository.com/handle/2436/625889©2020 The Authors. Published by Springer. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link: https://doi.org/10.1038/s41467-020-17449-1Duplication of mammalian genomes requires replisomes to overcome numerous impediments during passage through open (eu) and condensed (hetero) chromatin. Typically, studies of replication stress characterize mixed populations of challenged and unchallenged replication forks, averaged across S phase, and model a single species of "stressed" replisome. Here, in cells containing potent obstacles to replication, we find two different lesion proximal replisomes. One is bound by the DONSON protein and is more frequent in early S phase, in regions marked by euchromatin. The other interacts with the FANCM DNA translocase, is more prominent in late S phase, and favors heterochromatin. The two forms can also be detected in unstressed cells. ChIP-seq of DNA associated with DONSON or FANCM confirms the bias of the former towards regions that replicate early and the skew of the latter towards regions that replicate late.application/pdfenLicence for published version: Creative Commons Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/chromosomesreplisomeHela CellsEuchromatinHeterochromatinHumansDNA HelicasesCell Cycle ProteinsNuclear ProteinsS PhaseDNA Replication TimingChromatin Immunoprecipitation SequencingJournal article2041-1723Nature Communications