Baldwin, Timothy C.Handford, Michael G.Yuseff, Maria-IsabelOrellana, ArielDupree, Paul2008-01-292008-01-292001The Plant Cell, 13(10): 2283-22951040-46511159580210.1105/tpc.13.10.2283http://hdl.handle.net/2436/17052Metadata only, Full text available at links above.Transport of nucleotide sugars across the Golgi apparatus membrane is required for the luminal synthesis of a variety of plant cell surface components. We identified an Arabidopsis gene encoding a nucleotide sugar transporter (designated GONST1) that we have shown by transient gene expression to be localized to the Golgi. GONST1 complemented a GDP-mannose transport-defective yeast mutant (vrg4-2), and Golgi-rich vesicles from the complemented strain displayed increased GDP-mannose transport activity. GONST1 promoterenAmino Acid SequenceArabidopsisArabidopsis ProteinsBase SequenceCarrier ProteinsDNA PrimersGlycosylationGolgi ApparatusIntracellular MembranesMembrane Transport ProteinsMicroscopy, ConfocalMolecular Sequence DataProtein ConformationSequence AlignmentSequence Homology, Amino AcidIdentification and characterization of GONST1, a golgi-localized GDP-mannose transporter in Arabidopsis.Journal article