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Characterisation and carriage ratio of Clostridium difficile strains isolated from a community-dwelling elderly population in the United Kingdom
Miyajima, Fabio Swale, Andrew Price, Valerie Jones, Maureen Horan, Michael Beeching, Nicholas Brazier, Jonathan Parry, Christopher Pendleton, Neil
Miyajima, Fabio
Swale, Andrew
Price, Valerie
Jones, Maureen
Horan, Michael
Beeching, Nicholas
Brazier, Jonathan
Parry, Christopher
Pendleton, Neil
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2011-08-23
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Abstract
Background Community-associated Clostridium difficile infection (CDI) appears to be an increasing problem. Reported carriage rates by C.difficile are debatable with suggestions that primary asymptomatic carriage is associated with decreased risk of subsequent diarrhoea. However, knowledge of potential reservoirs and intestinal carriage rates in the community, particularly in the elderly, the most susceptible group, is limited. We have determined the presence of C.difficile in the faeces of a healthy elderly cohort living outside of long-term care facilities (LCFs) in the United Kingdom. Methods Faecal samples from 149 community-based healthy elderly volunteers (median age 81 years) were screened for C.difficile using direct (Brazier's CCEY) and enrichment (Cooked Meat broth) culture methods and a glutamate dehydrogenase (GDH) immunoassay. Isolates were PCR-ribotyped and analysed for toxin production and the presence of toxin genes. Results Of 149 faecal samples submitted, six (4%) were found to contain C.difficile. One particular sample was positive by both the GDH immunoassay and direct culture, and concurrently produced two distinct strain types: one toxigenic and the other non-toxigenic. The other five samples were only positive by enrichment culture method. Overall, four C.difficile isolates were non-toxigenic (PCR-ribotypes 009, 026 (n = 2) and 039), while three were toxigenic (PCR-ribotypes 003, 005 and 106). All individuals who had a positive culture were symptom-free and none of them had a history of CDI and/or antibiotics use in the 3 month period preceding recruitment. Conclusions To our knowledge, this is the first study of the presence of C.difficile in healthy elderly community-dwelling individuals residing outside of LCFs. The observed carriage rate is lower than that reported for individuals in LCFs and interestingly no individual carried the common epidemic strain PCR-ribotype 027 (NAP1/BI). Further follow-up of asymptomatic carriers in the community, is required to evaluate host susceptibility to CDI and identify dynamic changes in the host and microbial environment that are associated with pathogenicity.
Citation
Miyajima, F., Roberts, P., Swale, A., Price, V. et al. (2011) Characterisation and carriage ratio of Clostridium difficile strains isolated from a community-dwelling elderly population in the United Kingdom, PLoS ONE, 6(8): e22804. https://doi.org/10.1371/journal.pone.0022804
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Journal article
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en
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© 2011 The Authors. Published by Public Library of Science. This is an open access article available under a Creative Commons licence.
The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1371/journal.pone.0022804
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1932-6203
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1932-6203
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This work was supported by the Liverpool Biomedical Research Centre (Royal Liverpool University Hospital/NHS Trust and the Wolfson Centre for Personalised Medicine/University of Liverpool), the National Institute for Health Research and The Northwest Development Agency. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.