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Assessing label stability in oligopeptide-modified polymer filament for advanced materials: ultraviolet exposure and biodegradation study
Rydz, Joanna Duale, Khadar Musioł, Marta Janeczek, Henryk Hercog, Anna Marcinkowski, Andrzej Molnar, Kristof Michel, Frederick C. Klingman, Michael Focarete, Maria Letizia
Rydz, Joanna
Duale, Khadar
Musioł, Marta
Janeczek, Henryk
Hercog, Anna
Marcinkowski, Andrzej
Molnar, Kristof
Michel, Frederick C.
Klingman, Michael
Focarete, Maria Letizia
Authors
Rydz, Joanna
Duale, Khadar
Musioł, Marta
Janeczek, Henryk
Hercog, Anna
Marcinkowski, Andrzej
Molnar, Kristof
Michel, Frederick C.
Klingman, Michael
Focarete, Maria Letizia
Puskas, Judit E.
Mielczarek, Przemysław
Suder, Piotr
El Fray, Miroslawa
Walkowiak, Konrad
Rokicka, Joanna
Niedźwiedź, Malwina
Grundmann, Alexander
Kaysser, Simon T.
Detjen, Sönke
Johnston, Brian
Radecka, Iza
Kannappan, Vinodh
Kowalczuk, Marek
Duale, Khadar
Musioł, Marta
Janeczek, Henryk
Hercog, Anna
Marcinkowski, Andrzej
Molnar, Kristof
Michel, Frederick C.
Klingman, Michael
Focarete, Maria Letizia
Puskas, Judit E.
Mielczarek, Przemysław
Suder, Piotr
El Fray, Miroslawa
Walkowiak, Konrad
Rokicka, Joanna
Niedźwiedź, Malwina
Grundmann, Alexander
Kaysser, Simon T.
Detjen, Sönke
Johnston, Brian
Radecka, Iza
Kannappan, Vinodh
Kowalczuk, Marek
Editors
Other contributors
Epub Date
Issue Date
2025-09-02
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Abstract
This study explores the integration of triglycine, an oligopeptide, as a green molecular marker in 3D-printed poly(1,4-butylene adipate-co-1,4-butylene terephthalate)/polylactide (PBAT/PLA)-based specimens with different printing temperatures to enhance the traceability of (bio)degradable polymers. This approach supports the advancement of sustainable materials by enabling the identification of the material origin and degradation processes. The research assesses the behavior of the labeled polymer under UV exposure, evaluating the stability of the oligopeptide marker to ensure that the information remains retrievable even after exposure to environmental stressors. In addition, their behavior during aerobic composting, as well as anaerobic digestion, is investigated to promote environmentally friendly practices. This study employed an extraction procedure to isolate and retrieve encoded information, which was then analyzed using a mass spectrometry method, ESI/TIMS-Q-TOF. This makes it possible to determine the sequence of the oligopeptide and compare it with the previously used MALDI-TOF/TOF mass spectrometry procedure. Cytotoxicity studies were also conducted to assess the potential hazards associated with PBAT/PLA-based specimens, considering their potential biomedical applications. The PBAT/PLA-based specimens demonstrated good oligopeptide stability, enabling effective retrieval of recorded information from the green polymer/oligopeptide system even after UV exposure. UV irradiation affected cold crystallization temperature and melting temperature and caused self-chain/cross-linking of the PBAT/PLA-based specimens. In general, the analyses show that specimens printed at a higher temperature (190 °C) have a higher degradation rate than those printed at a lower temperature (155 °C). This phenomenon was attributed to the higher porosity and increased water permeability of the specimens printed at 190 °C, compared to those printed at 155 °C, which is likely due to the greater phase separation and reduced miscibility in the former.
Citation
Rydz, J., Duale, K., Musioł, M. et al. (2025) Assessing label stability in oligopeptide-modified polymer filament for advanced materials: ultraviolet exposure and biodegradation study, 13 (36), pp. 14873–14892
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PubMed ID
PubMed Central ID
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Type
Journal article
Language
en
Description
© 2025 The Authors. Published by ACS. This is an open access article available under a Creative Commons licence.
The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1021/acssuschemeng.5c04602
Series/Report no.
ISSN
2168-0485
EISSN
2168-0485
ISBN
ISMN
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Sponsors
This work was supported by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 872152, project GREEN-MAP, an international project cofinanced by the program of the Minister of Science and Higher Education entitled “PMW” in the years 2020–2024; contract No. 5092/H2020/2020/2, and Joint Polish-Romanian project under the agreement on scientific cooperation between the Polish Academy of Sciences and Romanian Academy of Sciences, “Design of cyclodextrin-polyester-amides for special applications”. Ms Nina Stefaniak is acknowledged for her help with the degradation tests. The authors also gratefully acknowledge funding from USDA-NIFA, Hatch project number OHO01417, and startup funds from the Ohio State University #11232011000-11-PUSKAS.