Loading...
Downregulation of aquaporin 3 inhibits cellular proliferation, migration and invasion in the MDA‑MB‑231 breast cancer cell line
Arif, Muhammad Kitchen, Philip Conner, Matthew Hill, Eric Nagel, David Bill, Roslyn Dunmore, Simon Armesilla, Angel Gross, Stephane Carmichael, Amtul
Arif, Muhammad
Kitchen, Philip
Conner, Matthew
Hill, Eric
Nagel, David
Bill, Roslyn
Dunmore, Simon
Armesilla, Angel
Gross, Stephane
Carmichael, Amtul
Editors
Other contributors
Affiliation
Epub Date
Issue Date
2018-05-21
Submitted date
Alternative
Abstract
Aquaporins are membrane proteins that regulate cellular water flow. Recently, aquaporins have been proposed as mediators of cancer cell biology. A subset of aquaporins, referred to as aquaglyceroporins are known to facilitate the transport of glycerol. The present study describes the effect of gene knockdown of the aquaglyceroporin AQP3 on MDA‑MB‑231 breast cancer cell proliferation, migration, invasion, adherence and response to the chemotherapeutic agent 5‑fluorouracil. shRNA mediated AQP3 gene knockdown induced a 28% reduction in cellular proliferation (P<0.01), a 39% decrease in migration (P<0.0001), a 24% reduction in invasion (P<0.05) and a 25% increase in cell death at 100 µM 5‑FU (P<0.01). Analysis of cell permeability to water and glycerol revealed that MDA‑MB‑231 cells with knocked down AQP3 demonstrated a modest decrease in water permeability (17%; P<0.05) but a more marked decrease in glycerol permeability (77%; P<0.001). These results suggest that AQP3 has a role in multiple aspects of breast cancer cell pathophysiology and therefore represents a novel target for therapeutic intervention.
Citation
Arif, M., Kitchen, P., Conner, M., Hill, E., Nagel, D., Bill, R., Dunmore, S., Armesilla, A., Gross, S., Carmichael, A., Conner, A., and Brown, J. (2018) 'Downregulation of aquaporin 3 inhibits cellular proliferation, migration and invasion in the MDA‑MB‑231 breast cancer cell line', Oncology Letters, 16 (1) pp. 713-720
Publisher
Journal
Research Unit
PubMed ID
PubMed Central ID
Embedded videos
Additional Links
Type
Journal article
Language
en
Description
Series/Report no.
ISSN
1792-1074