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Oxidized polyethylene wax as a potential carbon source for PHA production
Radecka, Iza Irorere, Victor Jiang, Guozhan Hill, David Williams, Craig Adamus, Grazyna Kwiecień, Michal Marek, Adam Zawadiak, Jan Johnston, Brian
Radecka, Iza
Irorere, Victor
Jiang, Guozhan
Hill, David
Williams, Craig
Adamus, Grazyna
Kwiecień, Michal
Marek, Adam
Zawadiak, Jan
Johnston, Brian
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2016-05-13
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Abstract
We report on the ability of bacteria to produce biodegradable polyhydroxyalkanoates (PHA) using oxidized polyethylene wax (O-PEW) as a novel carbon source. The O-PEW was obtained in a process that used air or oxygen as an oxidizing agent. R. eutropha H16 was grown for 48 h in either tryptone soya broth (TSB) or basal salts medium (BSM) supplemented with O-PEW and monitored by viable counting. Study revealed that biomass and PHA production was higher in TSB supplemented with O-PEW compared with TSB only. The biopolymers obtained were preliminary characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The detailed structural evaluation at the molecular level was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The study revealed that, when TSB was supplemented with O-PEW, bacteria produced PHA which contained 3-hydroxybutyrate and up to 3 mol % of 3-hydroxyvalerate and 3-hydroxyhexanoate co-monomeric units. The ESI-MS/MS enabled the PHA characterization when the content of 3-hydroxybutyrate was high and the appearance of other PHA repeating units was very low.
Citation
Radecka I., Irorere V., Jiang G., Hill D., Williams C., Adamus G., Kwiecień M., Marek AA., Zawadiak J., Johnston B., Kowalczuk M. (2016) 'Oxidized polyethylene wax as a potential carbon source for PHA production', Materials, 9 (5): 367
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Journal article
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en
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© 2016 The Authors. Published by MDPI. This is an open access article available under a Creative Commons licence.
The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.3390/ma9050367
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1996-1944