DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.

Duplication of mammalian genomes requires replisomes to overcome numerous impediments during passage through open (eu) and condensed (hetero) chromatin. Typically, studies of replication stress characterize mixed populations of challenged and unchallenged replication forks, averaged across S phase, and model a single species of "stressed" replisome. Here, in cells containing potent obstacles to replication, we find two different lesion proximal replisomes. One is bound by the DONSON protein and is more frequent in early S phase, in regions marked by euchromatin. The other interacts with the FANCM DNA translocase, is more prominent in late S phase, and favors heterochromatin. The two forms can also be detected in unstressed cells. ChIP-seq of DNA associated with DONSON or FANCM confirms the bias of the former towards regions that replicate early and the skew of the latter towards regions that replicate late.

Citation

Zhang, J., Bellani, M.A., James, R.C. et al. (2020) DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain. Nature Communications 11, 3951. https://doi.org/10.1038/s41467-020-17449-1

PubMed ID

32769987 (pubmed)

Additional Links

https://doi.org/10.1038/s41467-020-17449-1

Type

Journal article

Language

en

Description

©2020 The Authors. Published by Springer. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link: https://doi.org/10.1038/s41467-020-17449-1

ISSN

2041-1723

EISSN

2041-1723

Rights

Licence for published version: Creative Commons Attribution 4.0 International

cb

Collections

Faculty of Science and Engineering