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Smoking genes: a case–control study of dopamine transporter gene (SLC6A3) and dopamine receptor genes (DRD1, DRD2 and DRD3) polymorphisms and smoking behaviour in a Malay male cohort
Ruzilawati, Abu Bakar Muhamed, Siti Khariem Sophia Ahmad, Imran
Ruzilawati, Abu Bakar
Muhamed, Siti Khariem Sophia
Ahmad, Imran
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2020-12-03
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Abstract
Dopamine receptor and dopamine transporter genes polymorphisms have been associated with cigarette smoking behaviour in different populations. The aim of this case–control study was to evaluate polymorphisms in the dopamine transporter gene (SLC6A3 (rs27072)) and the dopamine receptor genes (DRD1 (rs686), DRD2 (rs1800497) and DRD3 (rs7653787)) and their contribution to smoking behaviour in a Malay male population. We identified 476 participants over the age of 18 years comprising 238 smokers and 238 non-smokers. Information such as age, height, weight, body mass index, systolic and diastolic blood pressures, marital status, and smoking status of close family members were taken. For the genetic study, we genotyped four genes (SLC6A3 (rs27072), DRD1 (rs686), DRD2 (rs1800497) and DRD3 (rs7653787)) using the polymerase chain reaction–restriction fragment length polymorphism method and further confirmed our findings with sequencing. Dopamine receptor genes (DRD1, DRD2 and DRD3) were found to be associated with smoking behaviour in a Malay male population. The dopamine transporter gene (SLC6A3) did not show this association. Significant differences were observed between smokers’ and non-smokers’ age, systolic blood pressure, marital status and family members who smoke. Smoking behaviour is significantly influenced by genetic variations of DRD1, DRD2 and DRD3 in a Malay male population.
Citation
Ruzilawati AB, Islam MA, Muhamed SKS, Ahmad I. Smoking Genes: A Case–Control Study of Dopamine Transporter Gene (SLC6A3) and Dopamine Receptor Genes (DRD1, DRD2 and DRD3) Polymorphisms and Smoking Behaviour in a Malay Male Cohort. Biomolecules. 2020; 10(12):1633. https://doi.org/10.3390/biom10121633
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PubMed ID
33287325 (pubmed)
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Journal article
Language
en
Description
© 2020 The Authors. Published by MDPI. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.3390/biom10121633
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2218-273X
EISSN
2218-273X
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This research was funded by the Universiti Sains Malaysia (USM) short term grant, 304/PPSP/61313050 and USM Research University grant, 1001/PPSP/8012256.
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Licence for published version: Creative Commons Attribution 4.0 International