Loading...
Variable alterations of the microbiota, without metabolic or immunological change, following faecal microbiota transplantation in patients with chronic pouchitis
Landy, J Walker, AW Li, JV Al-Hassi, Hafid Omar Ronde, E English, NR Mann, ER Bernardo, D McLaughlin, SD Parkhill, J
Landy, J
Walker, AW
Li, JV
Al-Hassi, Hafid Omar
Ronde, E
English, NR
Mann, ER
Bernardo, D
McLaughlin, SD
Parkhill, J
Editors
Other contributors
Affiliation
Epub Date
Issue Date
2015-08-12
Submitted date
Alternative
Abstract
Faecal microbiota transplantation (FMT) is effective in the treatment of Clostridium difficile infection, where efficacy correlates with changes in microbiota diversity and composition. The effects of FMT on recipient microbiota in inflammatory bowel diseases (IBD) remain unclear. We assessed the effects of FMT on microbiota composition and function, mucosal immune response, and clinical outcome in patients with chronic pouchitis. Eight patients with chronic pouchitis (current PDAI ‰7) were treated with FMT via nasogastric administration. Clinical activity was assessed before and four weeks following FMT. Faecal coliform antibiotic sensitivities were analysed, and changes in pouch faecal and mucosal microbiota assessed by 16S rRNA gene pyrosequencing and 1 H NMR spectroscopy. Lamina propria dendritic cell phenotype and cytokine profiles were assessed by flow cytometric analysis and multiplex assay. Following FMT, there were variable shifts in faecal and mucosal microbiota composition and, in some patients, changes in proportional abundance of species suggestive of a 'healthier' pouch microbiota. However, there were no significant FMT-induced metabolic or immunological changes, or beneficial clinical response. Given the lack of clinical response following FMT via a single nasogastric administration our results suggest that FMT/bacteriotherapy for pouchitis patients requires further optimisation.
Citation
Landy, J., Walker, A., Li, J. et al. (2015) Variable alterations of the microbiota, without metabolic or immunological change, following faecal microbiota transplantation in patients with chronic pouchitis. Scientific Reports, 5, 12955. https://doi.org/10.1038/srep12955
Publisher
Journal
Research Unit
PubMed ID
26264409 (pubmed)
PubMed Central ID
Embedded videos
Additional Links
Type
Journal article
Language
en
Description
© 2015 The Authors. Published by Springer Nature. This is an open access article available under a Creative Commons licence.
The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1038/srep12955
Series/Report no.
ISSN
2045-2322
EISSN
2045-2322
ISBN
ISMN
Gov't Doc #
Sponsors
Rights
Licence for published version: Creative Commons Attribution 4.0 International