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Differences in the on-and off-tumor microbiota between right-and left-sided colorectal cancer
Quraishi, Mohammed Nabil Dickson, Edward A Acheson, Austin G Beggs, Andrew D Al-Hassi, Hafid Omar
Quraishi, Mohammed Nabil
Dickson, Edward A
Acheson, Austin G
Beggs, Andrew D
Al-Hassi, Hafid Omar
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Epub Date
Issue Date
2021-05-20
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Abstract
This study aims to determine differences in the on-and off-tumor microbiota between patients with right-and left-sided colorectal cancer. Microbiome profiling of tumor and tumor-adjacent biopsies from patients with right-sided (n = 17) and left-sided (n = 7) colorectal adenocarcinoma was performed using 16S ribosomal RNA sequencing. Off-tumor alpha and beta diversity were significantly different between right-and left-sided colorectal cancer patients. However, no differences in on-tumor diversity were observed between tumor locations. Comparing the off-tumor microbiota showed the right colon to be enriched with species of the Lachnoclostridium, Selenomonas, and Ruminococcus genera. Whereas the left colon is enriched with Epsilonbacteraeota phylum, Campylobacteria class, and Pasteurellales and Campylobacterales orders, in contrast, the on-tumor microbiota showed relatively fewer differences in bacterial taxonomy between tumor sites, with left tumors being enriched with Methylophilaceae and Vadin BE97 families and Alloprevotella, Intestinibacter, Romboutsia, and Ruminococcus 2 genera. Patients with left-sided colorectal cancer had large taxonomic differences between their paired on-and off-tumor microbiota, while patients with right-sided colorectal cancer showed relatively fewer taxonomic differences. Collectively, this suggests that the right and left colon show distinctive bacterial populations; however, the presence of a colonic tumor leads to a more consistent microbiota between locations.
Citation
Phipps O, Quraishi MN, Dickson EA, Steed H, Kumar A, Acheson AG, Beggs AD, Brookes MJ, Al-Hassi HO. (2021) Differences in the On- and Off-Tumor Microbiota between Right- and Left-Sided Colorectal Cancer. Microorganisms, 9(5):1108. https://doi.org/10.3390/microorganisms9051108
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Research Unit
PubMed ID
34065545 (pubmed)
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Journal article
Language
en
Description
© 2021 The Authors. Published by MDPI. This is an open access article available under a Creative Commons licence.
The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.3390/microorganisms9051108
Series/Report no.
ISSN
2076-2607
EISSN
2076-2607
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Sponsors
This study represents independent research funded through the National Institute for Health Research (NIHR) Research for Patient Benefit (RfPB) program (grant number PB-PG-0110-21041).
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Licence for published version: Creative Commons Attribution 4.0 International