Loading...
Screening of a HUVEC cDNA library with transplant-associated coronary artery disease sera identifies RPL7 as a candidate autoantigen associated with this disease.
Linke, A.T. Marchant, B. Marsh, P. Frampton, Geoffrey Murphy, John J. Rose, Marlene L.
Linke, A.T.
Marchant, B.
Marsh, P.
Frampton, Geoffrey
Murphy, John J.
Rose, Marlene L.
Editors
Other contributors
Affiliation
Epub Date
Issue Date
2001
Submitted date
2007-01-24
Subjects
Alternative
Abstract
A HUVEC cDNA library was screened with sera from two patients who had developed transplant-associated coronary artery disease (TxCAD) following cardiac transplantation. A total of six positive clones were isolated from a primary screen of 40 000 genes. Subsequent DNA sequence analysis identified these to be lysyl tRNA synthetase, ribosomal protein L7, ribosomal protein L9, beta transducin and TANK. Another gene whose product could not be identified showed homology to a human cDNA clone (DKFZp566M063) derived from fetal kidney. Full-length constructs of selected genes were expressed as his-tag recombinant fusion proteins and used to screen a wider patient base by ELISA to determine prevalence and association with TxCAD. Of these ribosomal protein L7 showed the highest prevalence (55.6%) with TxCAD sera compared to 10% non-CAD.
Citation
Clinical & Experimental Immunology, 126(1): 173-179
Publisher
Journal
Research Unit
PubMed ID
11678915
PubMed Central ID
Embedded videos
Additional Links
Type
Journal article
Language
en
Description
Series/Report no.
ISSN
0009-9104