Elucidation of the mechanisms underlying the angiogenic effects of ginsenoside Rg(1) in vivo and in vitro.
Yue, Patrick Y. K. Wong, Daisy Y. L. Ha, Wai-Yan Fung, M.C. Mak, Nai Ki Yeung, H.W. Leung, Hei Wun Chan, Kelvin C. Liu, Liang Fan, T. P. David
Yue, Patrick Y. K.
Wong, Daisy Y. L.
Ha, Wai-Yan
Fung, M.C.
Mak, Nai Ki
Yeung, H.W.
Leung, Hei Wun
Chan, Kelvin C.
Liu, Liang
Fan, T. P. David
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2005
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Abstract
The major active constituents of ginseng are ginsenosides, and Rg(1) is a predominant compound of the total extract. Recent studies have demonstrated that Rg(1) can promote angiogenesis in vivo and in vitro. In this study, we used a DNA microarray technology to elucidate the mechanisms of action of Rg(1). We report that Rg(1) induces the proliferation of HUVECs, monitored using [(3)H]-thymidine incorporation and Trypan blue exclusion assays. Furthermore, Rg(1) (150-600 nM) also showed an enhanced tube forming inducing effect on the HUVEC. Rg(1) was also demonstrated to promote angiogenesis in an in vivo Matrigel plug assay, and increase endothelial sprouting in the ex vivo rat aorta ring assay. Differential gene expression profile of HUVEC following treatment with Rg(1) revealed the expression of genes related to cell adhesion, migration and cytoskeleton, including RhoA, RhoB, IQGAP1, CALM2, Vav2 and LAMA4. Our results suggest that Rg(1) can promote angiogenesis in multiple models, and this effect is partly due to the modulation of genes that are involved in the cytoskeletal dynamics, cell-cell adhesion and migration.
Citation
Angiogenesis, 8(3): 205-216
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Research Unit
PubMed ID
16328162
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Journal article
Language
en
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ISSN
0969-6970