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Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering
Antoun Reyad, Ayman Pavlov, Michael Y Tenson, Tanel Ehrenberg M, M åNs
Antoun Reyad, Ayman
Pavlov, Michael Y
Tenson, Tanel
Ehrenberg M, M åNs
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2004-03-19
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Abstract
Light scattering and standard stopped-flow techniques were used to monitor rapid association of ribosomal subunits during initiation of eubacterial protein synthesis. The effects of the initiation factors IF1, IF2, IF3 and buffer conditions on subunit association were studied along with the role of GTP in this process. The part of light scattering theory that is essential for kinetic measurements is high-lighted in the main text and a more general treatment of Rayleigh scattering from macromolecules is given in an appendix.
Citation
Antoun, A., Pavlov, M. Y., Tenson, T. and Ehrenberg, M. (2004) Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering, Biological Procedures Online, 6(1), pp. 35-54.
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15103398
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Journal article
Language
en
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1480-9222