Determination of oleanolic acid in Caulis clematidis armandii by HPLC
Qing, Lin-Sen Zhang, Hao Lu, Guang-Hua Chan, Kelvin C. Leung, Kelvin Sze-Yin Chen Chu, Sze-Yin
Qing, Lin-Sen
Zhang, Hao
Lu, Guang-Hua
Chan, Kelvin C.
Leung, Kelvin Sze-Yin
Chen Chu, Sze-Yin
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2006
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Abstract
OBJECTIVE: To develop an HPLC method for the content determination of oleanolic acid in Caulis clematidis armandii after hydrolysis. METHODS: In the system of HPLC, a Alltima C_(18)(250 mm×4.6 mm,5 μm) column was used with a mixture of methanol-water-acetic acid-triethylamine(89∶11∶0.04∶0.02) as the mobile phase.The measuring wavelength was set at 207 nm.The flow rate was 1.0 ml·min~(-1) and the column temperature maintained at 45 ℃.RESULTS The oleanolic acid peak in chromatograms was completely separated from ...
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West China Journal of Pharmaceutical Sciences, 21(3): 273-274
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Journal article
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zh
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1006-0103