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Age-related alterations in blood and colonic dendritic cell properties
Vora, R Bernardo, D Durant, L Reddi, D Hart, AL Fell, JME Al-Hassi, Hafid Omar Knight, SC
Vora, R
Bernardo, D
Durant, L
Reddi, D
Hart, AL
Fell, JME
Al-Hassi, Hafid Omar
Knight, SC
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2016-03-01
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Abstract
Background: Dendritic cells (DC) determine initiation, type and location of immune responses and, in adults, show decreased Toll-like receptors and some increased cytokine levels on ageing. Few studies in children have characterised DC or explored DC-related mechanisms producing age-related immune changes. Results: The pDC marker BDCA2 (but not CD123) was absent in pre-pubertal children and numbers of pDC decreased with age. Blood and colonic DC were more mature and activated in adults. Decrease in pDC numbers correlated with reduced GM-CSF levels with aging, but increasing IL-4 and IL-8 levels correlated with a more activated DC profile in blood. CXCL16 levels decreased with age. Methods: Blood and colonic DC phenotypes were determined in healthy adults and children by flow cytometry and correlated with aging. Blood DC were divided into plasmacytoid (pDC) and myeloid (mDC) while only mDC were identified in colon. Serum cytokine levels were determined by multiplex cytokine assays and correlated with DC properties. Conclusions: In children, lack of BDCA2, a receptor mediating antigen capture and inhibiting interferon induction, may be immunologically beneficial during immune development. Conversely, reduced pDC numbers, probably secondary to decreasing GM-CSF and increasing cytokine-induced maturation of DC are likely to determine deteriorating immunity with ageing.
Citation
Vora, R., Bernardo, D., Durant, L., Reddi, D., Hart, A.L., Fell, J.M.E., Al-Hassi, H.O. and Knight, S.C. (2016) Age-related alterations in blood and colonic dendritic cell properties, Oncotarget, 2016, 7, pp. 11913-11922. https://doi.org/10.18632/oncotarget.7799
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Research Unit
PubMed ID
26942871 (pubmed)
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Journal article
Language
en
Description
© 2016 The Authors. Published by Impact Journals. This is an open access article available under a Creative Commons licence.
The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.18632/oncotarget.7799
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ISSN
1949-2553
EISSN
1949-2553
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The authors gratefully acknowledge the support of the Biotechnology and Biological Sciences Research Council (BBSRC). This research was funded by the BBSRC Institute Strategic Programme for Gut Health and Food Safety BB/J004529/1. The authors also gratefully acknowledge the support of the Westminster Medical School Research Trust and Crohn’s in Childhood Research Association (Reference JRC SG 002 03/13-14)
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Licence for published version: Creative Commons Attribution 4.0 International