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Gene Loss and Lineage-Specific Restriction-Modification Systems Associated with Niche Differentiation in the Campylobacter jejuni Sequence Type 403 Clonal Complex
Morley, Laura McNally, Alan Paszkiewicz, Konrad Corander, Jukka Méric, Guillaume Sheppard, Samuel K. Blom, Jochen Manning, Georgina
Morley, Laura
McNally, Alan
Paszkiewicz, Konrad
Corander, Jukka
Méric, Guillaume
Sheppard, Samuel K.
Blom, Jochen
Manning, Georgina
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2015-05-05
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ABSTRACT Campylobacter jejuni is a highly diverse species of bacteria commonly associated with infectious intestinal disease of humans and zoonotic carriage in poultry, cattle, pigs, and other animals. The species contains a large number of distinct clonal complexes that vary from host generalist lineages commonly found in poultry, livestock, and human disease cases to host-adapted specialized lineages primarily associated with livestock or poultry. Here, we present novel data on the ST403 clonal complex of C. jejuni , a lineage that has not been reported in avian hosts. Our data show that the lineage exhibits a distinctive pattern of intralineage recombination that is accompanied by the presence of lineage-specific restriction-modification systems. Furthermore, we show that the ST403 complex has undergone gene decay at a number of loci. Our data provide a putative link between the lack of association with avian hosts of C. jejuni ST403 and both gene gain and gene loss through nonsense mutations in coding sequences of genes, resulting in pseudogene formation.
Citation
Morley, L., McNally, A., Paszkiewicz, K., Corander, J. et al (2015) Gene Loss and Lineage-Specific Restriction-Modification Systems Associated with Niche Differentiation in the Campylobacter jejuni Sequence Type 403 Clonal Complex, Applied and Environmental Microbiology, 81 (11), pp. 3641-3647
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Journal article
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en
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This is an accepted manuscript of an article published by American Society for Microbiology in Applied and Environmental Microbiology on 05/05/2015, available online: https://doi.org/10.1128/AEM.00546-15
The accepted version of the publication may differ from the final published version.
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0099-2240
1098-5336
1098-5336
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L.M. was funded by a Nottingham Trent University Ph.D. studentship. A.M. is supported by the Royal Society (IE121459). S.K.S. is supported by a Wellcome Trust Career Development Fellowship, with additional funding from the BBSRC (BB/I02464X/1) and MRC-CLIMB (MR/L015080/1). J.C. is supported by ERC grant 239784 and AoF grant 251170. DNA sequencing was carried out at the Exeter Sequencing Service, which is supported by the following grants: Wellcome Trust Institutional Strategic Support Fund WT097835MF, Wellcome Trust Multi User Equipment Award WT101650MA, and BBSRC LOLA award BB/K003240/1.