Loading...
Bionic cartilage acellular matrix microspheres as scaffold for engineering cartilage
Liu, Jun Yu, Cheng Lu, Gonggong Tang, James Zhenggui Wang, Yonghui Zhang, Boqing Sun, Yong Lin, Hai Wang, Qiguang Liang, Jie
Liu, Jun
Yu, Cheng
Lu, Gonggong
Tang, James Zhenggui
Wang, Yonghui
Zhang, Boqing
Sun, Yong
Lin, Hai
Wang, Qiguang
Liang, Jie
Editors
Other contributors
Affiliation
Epub Date
Issue Date
2018-12-12
Submitted date
Files
Alternative
Abstract
Extracellular matrix (ECM) scaffolds made from decellularized natural cartilage have been successfully used in cartilage lesion repair, but allogeneic cartilage donors are always in shortage and xenogeneic cartilage tissues may have the risk of unknown disease transfer. In this study, we constructed artificial bionic cartilage microspheres by encapsulating MSCs in collagen microspheres and cultured in a chondrogenic-inducing medium. Then, acellular matrix microsphere (BCAMM) scaffolds were fabricated from the cultured microspheres at three different developmental stages. A novel technique was introduced to fabricate BCAMM scaffolds, which enabled the production and utilization of the scaffolds in a short time. Due to the differences in surface morphologies and biological compositions, the three BCAMM scaffolds showed different chondrogenic effects. The 10-day BCAMM (10-BCAMM) scaffold showed the best overall results, successfully inducing MSC chondrogenesis without any additional fetal bovine serum or induction components (TGF-β or dexamethasone). In comparison, the 5-day BCAMM (5-BCAMM) scaffold showed potential osteogenic effects. The advantages of micron-sized BCAMMs are outlined, specifically in the easier decellularization process without grinding, homogeneous cell seeding and infiltration, chondrogenic induction and better fitting to the irregular lesion shape.
Citation
Liu, J. (2018) 'Bionic cartilage acellular matrix microspheres as scaffold for engineering cartilage', Journal of Materials Chemistry B. 7(4)
doi: 10.1039/C8TB02999G
Publisher
Journal
Research Unit
PubMed ID
PubMed Central ID
Embedded videos
Type
Journal article
Language
en
Description
Series/Report no.
ISSN
2050-750X
EISSN
ISBN
ISMN
Gov't Doc #
Sponsors
Rights
Attribution-NonCommercial-NoDerivs 3.0 United States