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Mass spectrometry reveals molecular structure of polyhydroxyalkanoates attained by bioconversion of oxidized polypropylene waste fragments
Johnston, Brian Chiellini, Emo Barsi, David Ilieva, Vassilka Ivanova Sikorska, Wanda Musioł, Marta Zięba, Magdalena Chaber, Paweł Marek, Adam A
Johnston, Brian
Chiellini, Emo
Barsi, David
Ilieva, Vassilka Ivanova
Sikorska, Wanda
Musioł, Marta
Zięba, Magdalena
Chaber, Paweł
Marek, Adam A
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2019-09-27
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Abstract
This study investigated the molecular structure of the polyhydroxyalkanoate (PHA) produced via a microbiological shake flask experiment utilizing oxidized polypropylene (PP) waste as an additional carbon source. The bacterial strain Cupriavidus necator H16 was selected as it is non-pathogenic, genetically stable, robust, and one of the best known producers of PHA. Making use of PHA oligomers, formed by controlled moderate-temperature degradation induced by carboxylate moieties, by examination of both the parent and fragmentation ions, the ESI-MS/MS analysis revealed the 3-hydroxybutyrate and randomly distributed 3-hydroxyvalerate as well as 3-hydroxyhexanoate repeat units. Thus, the bioconversion of PP solid waste to a value-added product such as PHA tert-polymer was demonstrated.
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Johnston, B.; Radecka, I.; Chiellini, E.; Barsi, D.; Ilieva, V.I.; Sikorska, W.; Musioł, M.; Zięba, M.; Chaber, P.; Marek, A.A.; Mendrek, B.; Ekere, A.I.; Adamus, G.; Kowalczuk, M. Mass Spectrometry Reveals Molecular Structure of Polyhydroxyalkanoates Attained by Bioconversion of Oxidized Polypropylene Waste Fragments. Polymers 2019, 11 (10), pp. 1580.
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en
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2073-4360
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2073-4360
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This research was funded by the Research Investment Fund, University of Wolverhampton, Faculty of Science and Engineering, UK. This work was also partially supported the European Regional Development Fund Project EnTRESS No 01R16P00718 and the PELARGODONT Project UM0-2016/22/Z/STS/00692 financed under the M-ERA.NET 2 Program of Horizon 2020.