Peptide Synthesis and Applications
Howl, John D.
Howl, John D.
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2005
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Abstract
Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included. (Humana Press)
Citation
Methods in Molecular Biology, 298
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Authored book
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en
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This title is published as a book but is also in a journal series
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ISSN
1064-3745
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ISBN
978-1-58829-317-6
978-1-59259-877-9
978-1-59259-877-9