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dc.contributor.authorLu, Guang-Hua
dc.contributor.authorChan, Kelvin C.
dc.contributor.authorLiang, Yi-Zeng
dc.contributor.authorLeung, Kelvin Sze-Yin
dc.contributor.authorChan, Chi-Leung
dc.contributor.authorJiang, Zhi-Hong
dc.contributor.authorZhao, Zhong-Zhen
dc.date.accessioned2007-03-07T14:50:34Z
dc.date.available2007-03-07T14:50:34Z
dc.date.issued2005
dc.date.submitted2007-02-07
dc.identifier.citationJournal of Chromatography A, 1073(1-2): 383-392
dc.identifier.issn0021-9673
dc.identifier.pmid15909545
dc.identifier.doi10.1016/j.chroma.2004.11.080
dc.identifier.urihttp://hdl.handle.net/2436/9849
dc.description.abstractA high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures.
dc.format.extent359273 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherElsevier
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG8-4F1GYXY-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=3309f2e3b34844ab2c0881de1cb6f789
dc.subjectAngelica spp
dc.subjectLigusticum chuanxiong
dc.subjectCnidium officinale
dc.subjectUmbelliferae herbs
dc.subjectChromatographic fingerprints
dc.titleDevelopment of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs.
dc.typeJournal article
dc.format.digYES
refterms.dateFOA2018-08-22T07:15:13Z
html.description.abstractA high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures.


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