Characterisation of epitopes of pan-IgG/anti-G3m(u) and anti-Fc monoclonal antibodies.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsNelson, Paul N.
Westwood, Olwyn M. R.
Baumforth, Karl R. N.
Roden, Denise A.
Hay, Frank C.
MetadataShow full item record
AbstractThe characterisation of monoclonal antibodies (MAbs) and their epitopes is important prior to their application as molecular probes. In this study, Western blotting using IgG1 Fc and pFc' fragments was employed to screen seven MAbs before pepscan analysis to determine their reactivity to potentially linear epitopes. MAbs PNF69C, PNF110A, X1A11 and MAbs WC2, G7C, JD312, 1A1 detected epitopes within the C(H)3 and C(H)2 domains, respectively. However, only four MAbs showed pepscan profiles that highlighted likely target residues. In particular, MAbs PNF69C and PNF110A that have previously been characterised with pan-IgG and anti-G3m(u) specificity, detected the peptide motif 338-KAKGQPR-344 which was located within the N-terminal region of the C(H)3 domain. Furthermore the majority of residues were present in all four IgG subclasses. Consequently the peptide identified was consistent with the pan-IgG nature of these antibodies. By using PCImdad, a molecular display programme, this sequence was visualised as surface accessible, located in the C(H)2/C(H)3 inter-domain region and proximal to the residue arginine(435). It is speculated that this residue may be important for phenotypic expression of G3m(u) and specificity of these reagents. Pepscan analysis of MAbs G7C and JD312 (both pan-IgG) highlighted the core peptide sequence 290-KPREE-294, which was present in the C(H)2 domain and was common to all four IgG subclasses. PCImdad also showed this region to be highly accessible and was consistent with previous bioinformatic and autoimmune analysis of IgG. Overall these MAbs may serve as useful anti-IgG or anti-G3m(u) reagents and probes of immunoglobulin structure.
CitationImmunology Letters, 88(1): 77-83
- Characterisation of putative monoclonal anti-G3m(u) and anti-G3m(g) reagents and their antigenic determinants.
- Authors: Nelson PN, Goodall M, Jefferis R
- Issue date: 1994 Jan
- Monoclonal anti-equine IgE antibodies with specificity for different epitopes on the immunoglobulin heavy chain of native IgE.
- Authors: Wagner B, Radbruch A, Rohwer J, Leibold W
- Issue date: 2003 Mar 20
- Studies of human anti-IgM anti-IgG cryoglobulins. I. Patterns of reactivity with autologous and isologous human IgG and its subunits.
- Authors: Johnston SL, Abraham GN
- Issue date: 1979 Apr
- Monoclonal antibodies against heavy and light chains of domestic mink IgG.
- Authors: Peremislov VV, Mechetina LV, Taranin AV
- Issue date: 1992 Oct
- Immunogenic and antigenic epitopes of immunoglobulins--VII. The topographical distribution of Fc gamma epitopes and the relationship of an iso-allotypic specificity to the presence of histidine 435.
- Authors: Nik Jaafar MI, Lowe JA, Ling NR, Jefferis R
- Issue date: 1984 Feb