Plasma membrane Ca2+ ATPase 4 is required for sperm motility and male fertility
Cartwright, Elizabeth J.
Williams, Judith C.
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AbstractCalcium and Ca(2+)-dependent signals play a crucial role in sperm motility and mammalian fertilization, but the molecules and mechanisms underlying these Ca(2+)-dependent pathways are incompletely understood. Here we show that homozygous male mice with a targeted gene deletion of isoform 4 of the plasma membrane calcium/calmodulin-dependent calcium ATPase (PMCA), which is highly enriched in the sperm tail, are infertile due to severely impaired sperm motility. Furthermore, the PMCA inhibitor 5-(and-6)-carboxyeosin diacetate succinimidyl ester reduced sperm motility in wild-type animals, thus mimicking the effects of PMCA4 deficiency on sperm motility and supporting the hypothesis of a pivotal role of the PMCA4 on the regulation of sperm function and intracellular Ca(2+) levels.
CitationSchuh, K., Cartwright, E.J., Jankevics, E. et al. (2004) Plasma membrane Ca2+ ATPase 4 is required for sperm motility and male fertility. Journal of Biological Chemistry, 279(27), pp. 28220-28226.
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