Glucose induces and leptin decreases expression of uncoupling protein-2 mRNA in human islets.

Brown, James E. P.; Thomas, Steven; Digby, Janet E.; Dunmore, Simon J.

dc.contributor.author	Brown, James E. P.
dc.contributor.author	Thomas, Steven
dc.contributor.author	Digby, Janet E.
dc.contributor.author	Dunmore, Simon J.
dc.date.accessioned	2006-11-30T16:40:19Z
dc.date.available	2006-11-30T16:40:19Z
dc.date.issued	2002
dc.date.submitted	2006-11-30
dc.identifier.citation	FEBS Letters, 513(2-3): 189-192
dc.identifier.issn	0014-5793
dc.identifier.pmid	11904148
dc.identifier.doi	10.1016/S0014-5793(02)02296-2
dc.identifier.uri	http://hdl.handle.net/2436/6313
dc.description.abstract	Elevated islet uncoupling protein-2 (UCP-2) impairs beta-cell function and UCP-2 may be increased in clinical obesity and diabetes. We investigated the effects of glucose and leptin on UCP-2 expression in isolated human islets. Human islets were incubated for 24 h with glucose (5.5-22 mmol/l)+/-leptin (0-10 nmol/l). Some islet batches were incubated at high (22 mmol/l), and subsequently lower (5.5 mmol/l), glucose to assess reversibility of effects. Leptin effects on insulin release were also measured. Glucose dose-dependently increased UCP-2 expression in all islet batches, maximally by three-fold. This was not fully reversed by subsequently reduced glucose levels. Leptin decreased UCP-2 expression by up to 75%, and maximally inhibited insulin release by 47%, at 22 mmol/l glucose. This is the first report of UCP-2 expression in human islets and provides novel evidence of its role in the loss of beta-cell function in diabetes.
dc.format.extent	160852 bytes
dc.format.mimetype	application/pdf
dc.language.iso	en
dc.publisher	Elsevier BV
dc.relation.url	http://www.ingentaconnect.com/content/els/00145793/2002/00000513/00000002/art02296
dc.subject	Gene Expression
dc.subject	Glucose toxicity
dc.subject	Type 2 diabetes
dc.subject	β-Cell
dc.title	Glucose induces and leptin decreases expression of uncoupling protein-2 mRNA in human islets.
dc.type	Journal article
dc.format.dig	YES
refterms.dateFOA	2018-08-21T15:37:18Z
html.description.abstract	Elevated islet uncoupling protein-2 (UCP-2) impairs beta-cell function and UCP-2 may be increased in clinical obesity and diabetes. We investigated the effects of glucose and leptin on UCP-2 expression in isolated human islets. Human islets were incubated for 24 h with glucose (5.5-22 mmol/l)+/-leptin (0-10 nmol/l). Some islet batches were incubated at high (22 mmol/l), and subsequently lower (5.5 mmol/l), glucose to assess reversibility of effects. Leptin effects on insulin release were also measured. Glucose dose-dependently increased UCP-2 expression in all islet batches, maximally by three-fold. This was not fully reversed by subsequently reduced glucose levels. Leptin decreased UCP-2 expression by up to 75%, and maximally inhibited insulin release by 47%, at 22 mmol/l glucose. This is the first report of UCP-2 expression in human islets and provides novel evidence of its role in the loss of beta-cell function in diabetes.