Pharmacological targeting of the GABAB receptor alters Drosophila's behavioural responses to alcohol

Ranson, Daniel C; Ayoub, Samir S.; Corcoran, Olivia; Casalotti, Stefano O

dc.contributor.author	Ranson, Daniel C
dc.contributor.author	Ayoub, Samir S.
dc.contributor.author	Corcoran, Olivia
dc.contributor.author	Casalotti, Stefano O
dc.date.accessioned	2022-03-09T12:29:12Z
dc.date.available	2022-03-09T12:29:12Z
dc.date.issued	2020-02-13
dc.identifier.citation	Ranson, D.C., Ayoub, S.S., Corcoran, O., Casalotti, S.O. (2020) Pharmacological targeting of the GABAB receptor alters Drosophila's behavioural responses to alcohol. Addiction Biology. 2020;25 https://doi.org/10.1111/adb.12725	en
dc.identifier.issn	1355-6215	en
dc.identifier.pmid	30761704 (pubmed)
dc.identifier.doi	10.1111/adb.12725	en
dc.identifier.uri	http://hdl.handle.net/2436/624640
dc.description	© 2020 The Authors. Published by Wiley. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1111/adb.12725	en
dc.description.abstract	When exposed to ethanol, Drosophila melanogaster display a variety of addiction-like behaviours similar to those observed in mammals. Sensitivity to ethanol can be quantified by measuring the time at which 50% of the flies are sedated by ethanol exposure (ST50); an increase of ST50 following multiple ethanol exposures is widely interpreted as development of tolerance to ethanol. Sensitivity and tolerance to ethanol were measured after administration of the gamma-aminobutyric acid receptor B (GABAB) agonist (SKF 97541) and antagonist (CGP 54626), when compared with flies treated with ethanol alone. Dose-dependent increases and decreases in sensitivity to ethanol were observed for both the agonist and antagonist respectively. Tolerance was recorded in the presence of GABAB drugs, but the rate of tolerance development was increased by SKF 97451 and unaltered in presence of CGP 54626. This indicates that the GABAB receptor contributes to both the sensitivity to ethanol and mechanisms by which tolerance develops. The data also reinforce the usefulness of Drosophila as a model for identifying the molecular components of addictive behaviours and for testing drugs that could potentially be used for the treatment of alcohol use disorder (AUD).	en
dc.description.sponsorship	This work was carried out at the University of East London with the support of a PhD Studentship awarded to DCR from the Society for the Study of Addiction.	en
dc.format	application/pdf	en
dc.language	eng
dc.language.iso	en	en
dc.publisher	Wiley	en
dc.relation.url	https://onlinelibrary.wiley.com/doi/10.1111/adb.12725	en
dc.rights	Licence for published version: Creative Commons Attribution 4.0 International
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	addiction behaviours	en
dc.subject	alcohol use disorder	en
dc.subject	CGP 54626	en
dc.subject	GABA(B) receptor	en
dc.subject	SKF 97541	en
dc.subject	drosophila melanogaster	en
dc.title	Pharmacological targeting of the GABAB receptor alters Drosophila's behavioural responses to alcohol	en
dc.type	Journal article	en
dc.identifier.eissn	1369-1600
dc.identifier.journal	Addiction Biology	en
dc.date.updated	2022-03-09T07:22:23Z
dc.contributor.institution	Medicines Research Group, University of East London, London, UK.
dc.identifier.articlenumber	e12725
pubs.place-of-publication	United States
dc.date.accepted	2019-01-17
rioxxterms.funder	society for the study of addiction	en
rioxxterms.identifier.project	2017 PhD Studentship	en
rioxxterms.version	VoR	en
rioxxterms.licenseref.uri	http://creativecommons.org/licenses/by/4.0/	en
rioxxterms.licenseref.startdate	2022-03-09	en
dc.source.volume	25
dc.source.issue	2
dc.description.version	Published version
refterms.dateFCD	2022-03-09T12:29:02Z
refterms.versionFCD	VoR
refterms.dateFOA	2022-03-09T12:29:13Z