The investigation of thymol formulations containing poloxamer 407 and hydroxypropyl methylcellulose to inhibit candida biofilm formation and demonstrate improved bio-compatibility
dc.contributor.author | Al-Ani, Enas | |
dc.contributor.author | Heaselgrave, Wayne | |
dc.date.accessioned | 2022-01-19T14:42:39Z | |
dc.date.available | 2022-01-19T14:42:39Z | |
dc.date.issued | 2022-01-05 | |
dc.identifier.citation | Al-Ani, E. and Heaselgrave, W. (2022) The Investigation of Thymol Formulations Containing Poloxamer 407 and Hydroxypropyl Methylcellulose to Inhibit Candida Biofilm Formation and Demonstrate Improved Bio-Compatibility. Pharmaceuticals. 2022; 15(1):71. https://doi.org/10.3390/ph15010071 | en |
dc.identifier.issn | 1424-8247 | en |
dc.identifier.doi | 10.3390/ph15010071 | en |
dc.identifier.uri | http://hdl.handle.net/2436/624553 | |
dc.description | © 2022 The Authors. Published by MDPI. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.3390/ph15010071 | en |
dc.description.abstract | The aim of this study was to investigate the potential of thymol to inhibit Candida biofilm formation and improve thymol biocompatibility in the presence of hydroxypropyl methylcellulose (HPMC) and poloxamer 407 (P407), as possible drug carriers. Thymol with and without polymers were tested for its ability to inhibit biofilm formation, its effect on the viability of biofilm and biocompatibility studies were performed on HEK 293 (human embryonic kidney) cells. Thymol showed a concentration dependent biofilm inhibition; this effect was slightly improved when it was combined with HPMC. The Thymol-P407 combination completely inhibited the formation of biofilm and the antibiofilm effect of thymol decreased as the maturation of Candida biofilms increased. The effect of thymol on HEK 293 cells was a loss of nearly 100% in their viability at a concentration of 250 mg/L. However, in the presence of P407, the viability was 25% and 85% using neutral red uptake and sulforhodamine B assays, respectively. While, HPMC had less effect on thymol activity the thymol-P407 combination showed a superior inhibitory effect on biofilm formation and better biocompatibility with human cell lines. The combination demonstrates a potential medical use for the prevention of Candida biofilm formation. | en |
dc.format | application/pdf | en |
dc.language | en | |
dc.language.iso | en | en |
dc.publisher | MDPI | en |
dc.relation.url | https://www.mdpi.com/1424-8247/15/1/71 | en |
dc.subject | thymol | en |
dc.subject | hydroxypropyl methyl cellulose | en |
dc.subject | poloxamer 407 | en |
dc.subject | Candida albicans | en |
dc.subject | biofilm inhibition | en |
dc.subject | antibiofilm | en |
dc.subject | biocompatibility | en |
dc.title | The investigation of thymol formulations containing poloxamer 407 and hydroxypropyl methylcellulose to inhibit candida biofilm formation and demonstrate improved bio-compatibility | en |
dc.type | Journal article | en |
dc.identifier.eissn | 1424-8247 | |
dc.identifier.journal | Pharmaceuticals | en |
dc.date.updated | 2022-01-18T18:51:53Z | |
dc.date.accepted | 2021-12-31 | |
rioxxterms.funder | University of Wolverhampton | en |
rioxxterms.identifier.project | UOW19012022EA | en |
rioxxterms.version | VoR | en |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0/ | en |
rioxxterms.licenseref.startdate | 2022-10-19 | en |
dc.source.volume | 15 | |
dc.source.issue | 1 | |
dc.source.beginpage | 71 | |
dc.source.endpage | 71 | |
dc.description.version | Published online | |
refterms.dateFCD | 2022-01-19T14:41:33Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2022-01-19T14:42:40Z |