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dc.contributor.authorSchmerer, Wera M
dc.date.accessioned2021-12-09T15:02:27Z
dc.date.available2021-12-09T15:02:27Z
dc.date.issued2021-10-08
dc.identifier.citationSchmerer, W.M. (2021) Optimized Protocol for Chelex-based Extraction of DNA from Historical Skeletal Remains and Forensic Trace Samples. Protocol Exchange (2010) DOI: 10.21203/rs.3.pex-1652/v1en
dc.identifier.issn2043-0116en
dc.identifier.doi10.21203/rs.3.pex-1652/v1en
dc.identifier.urihttp://hdl.handle.net/2436/624473
dc.description© 2021 The Author. Published by Research Square. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://protocolexchange.researchsquare.com/article/pex-1652/v1en
dc.description.abstractPCR-based analysis of skeletonized human remains is a common aspect in both forensic human identification as well as Ancient DNA research. In this, both areas not merely utilize very similar methodology, but also share the same problems regarding quantity and quality of recovered DNA and presence of inhibitory substances in samples from excavated remains. To enable amplification based analysis of the remains, development of optimized DNA extraction procedures is thus a critical factor in both areas. The method paper here presents an optimized protocol for DNA extraction from ancient skeletonized remains using Chelex-100, with improved effectively in yielding amplifiable extracts from sample material excavated after centuries in a soil environment, which consequently have high inhibitor content and overall limited DNA preservation. Further studies showed that the optimized protocol can likewise be utilized for extraction of DNA from common and trace Forensic sample material.en
dc.formatapplication/pdfen
dc.languageEnglish
dc.language.isoenen
dc.publisherResearch Squareen
dc.relation.urlhttps://protocolexchange.researchsquare.com/article/pex-1652/v1en
dc.subjectdegraded DNAen
dc.subjectskeletal remainsen
dc.subjectDNA extractionen
dc.subjectChelex-100en
dc.subjectPCR inhibitorsen
dc.subjectmethod optimizationen
dc.subjectSTR-genotypingen
dc.subjectancient DNA researchen
dc.subjecthuman identificationen
dc.subjecttrace DNAen
dc.subjectancient DNAen
dc.titleOptimized protocol for Chelex-based extraction of DNA from historical skeletal remains and forensic trace samplesen
dc.typeOtheren
dc.identifier.journalProtocol Exchangeen
dc.date.updated2021-12-03T19:05:50Z
dc.date.accepted2021-10-08
rioxxterms.funderuniversity of wolverhamptonen
rioxxterms.identifier.projectUOW09122021WSen
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2021-12-09en
refterms.dateFCD2021-12-09T15:02:18Z
refterms.versionFCDVoR
refterms.dateFOA2021-12-09T15:02:28Z


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