Optimized protocol for Chelex-based extraction of DNA from historical skeletal remains and forensic trace samples
Abstract
PCR-based analysis of skeletonized human remains is a common aspect in both forensic human identification as well as Ancient DNA research. In this, both areas not merely utilize very similar methodology, but also share the same problems regarding quantity and quality of recovered DNA and presence of inhibitory substances in samples from excavated remains. To enable amplification based analysis of the remains, development of optimized DNA extraction procedures is thus a critical factor in both areas. The method paper here presents an optimized protocol for DNA extraction from ancient skeletonized remains using Chelex-100, with improved effectively in yielding amplifiable extracts from sample material excavated after centuries in a soil environment, which consequently have high inhibitor content and overall limited DNA preservation. Further studies showed that the optimized protocol can likewise be utilized for extraction of DNA from common and trace Forensic sample material.Citation
Schmerer, W.M. (2021) Optimized Protocol for Chelex-based Extraction of DNA from Historical Skeletal Remains and Forensic Trace Samples. Protocol Exchange (2010) DOI: 10.21203/rs.3.pex-1652/v1Publisher
Research SquareJournal
Protocol ExchangeAdditional Links
https://protocolexchange.researchsquare.com/article/pex-1652/v1Type
OtherLanguage
enDescription
© 2021 The Author. Published by Research Square. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://protocolexchange.researchsquare.com/article/pex-1652/v1ISSN
2043-0116ae974a485f413a2113503eed53cd6c53
10.21203/rs.3.pex-1652/v1
Scopus Count
Collections
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/