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dc.contributor.authorSchmerer, Wera Margarete
dc.date.accessioned2021-12-06T11:00:37Z
dc.date.available2021-12-06T11:00:37Z
dc.date.issued2021-09-18
dc.identifier.citationSchmerer W.M. (2021) Optimized protocol for DNA extraction from ancient skeletal human remains using Chelex-100. International Journal of Forensic Medicine, 3(1), pp. 18-22. DOI: 10.33545/27074447.2021.v3.i1a.35en
dc.identifier.issn2707-4447en
dc.identifier.doi10.33545/27074447.2021.v3.i1a.35en
dc.identifier.urihttp://hdl.handle.net/2436/624469
dc.description©2021 The Author. Published by AkiNik Publications. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://www.forensicpaper.com/archives/2021.v3.i1.A.35en
dc.description.abstractPCR-based analysis of skeletonized human remains is a common aspect in both forensic human identification as well as Ancient DNA research. In this, both areas not merely utilize very similar methodology, but also share the same problems regarding quantity and quality of recovered DNA and presence of inhibitory substances in samples from excavated remains. To enable amplification based analysis of the remains, development of optimized DNA extraction procedures is thus a critical factor in both areas. The study here presents an optimized protocol for DNA extraction from ancient skeletonized remains using Chelex-100, which proved to be effective in yielding amplifiable extracts from sample material excavated after centuries in a soil environment, which consequently have high inhibitor content and overall limited DNA preservation. Success of the optimization strategies utilized is shown in significantly improved amplification outcomes compared to the predecessor method.en
dc.formatapplication/pdfen
dc.languageEnglish
dc.language.isoenen
dc.publisherAkiNik Publicationsen
dc.relation.urlhttps://www.forensicpaper.com/archives/2021.v3.i1.A.35en
dc.subjectdegraded DNAen
dc.subjectskeletal remainsen
dc.subjectDNA extractionen
dc.subjectChelex-100en
dc.subjectPCR inhibitorsen
dc.subjectoptimizationen
dc.subjectSTR-genotypingen
dc.subjecthuman identificationen
dc.subjectancient DNAen
dc.titleOptimized protocol for DNA extraction from ancient skeletal human remains using Chelex-100en
dc.typeJournal articleen
dc.identifier.eissn2707-4455
dc.identifier.journalInternational Journal of Forensic Medicineen
dc.date.updated2021-12-03T18:57:56Z
dc.date.accepted2021-09-16
rioxxterms.funderUniversity of wolverhampton
rioxxterms.identifier.projectUOW06122021WSen
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc/4.0/en
rioxxterms.licenseref.startdate2021-12-06en
dc.source.volume3(1)
refterms.dateFCD2021-12-06T10:59:14Z
refterms.versionFCDVoR
refterms.dateFOA2021-12-06T11:00:38Z


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