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dc.contributor.authorHowl, John
dc.contributor.authorSilva, Joana
dc.contributor.authorFardilha, Margarida
dc.contributor.authorJones, Sarah
dc.contributor.editorLangel, Ulo
dc.date.accessioned2021-09-21T11:33:05Z
dc.date.available2021-09-21T11:33:05Z
dc.date.issued2021-11-12
dc.identifier.citationHowl J., Silva J.V., Fardilha M., Jones S. (2022) Protein Mimicry and the Design of Bioactive Cell-Penetrating Peptides: The Genesis of STOPSPERM Bioportides. In: Langel Ü. (eds) Cell Penetrating Peptides. Methods in Molecular Biology, vol 2383. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1752-6_20en
dc.identifier.isbn9781071617519en
dc.identifier.doi10.1007/978-1-0716-1752-6_20
dc.identifier.urihttp://hdl.handle.net/2436/624352
dc.descriptionThis is an accepted manuscript of a chapter published by Springer in Cell Penetrating Peptides: Methods and Protocols, available online: https://www.springer.com/gp/book/9781071617519 The accepted version of the publication may differ from the final published version. Please consult publisher policy on re-use.en
dc.description.abstractThe mature spermatozoon, a highly differentiated cell equipped for the sole purpose of fertilisation, lacks the protein machinery required for conventional endocytotic mechanisms. Perhaps contrary to expectation, cell penetrating peptides (CPPs) rapidly translocate across the unique sperm plasma membrane to accrete within distinct intracellular compartments. Confocal microscopy, employing red-fluorescent CPPs and bioportides, is a convenient platform to study this membrane translocation process. In the virtual absence of genetic expression, rapid physiological responses of human sperm are dependent upon protein-protein interactions that may be regulated by post-translational modifications including phosphorylation. This chapter provides an outline of the design of bioactive CPPs, or bioportides, which include protein-mimetic sequences from the interaction domains of sperm proteins. Protocols are included which enable the biological assessment of the impact of bioportides upon the viability and motility of spermatozoa.en
dc.formatapplication/pdfen
dc.language.isoenen
dc.publisherHumanaen
dc.relation.ispartofseriesMethods in Molecular Biology, 2383en
dc.relation.urlhttps://www.springer.com/gp/book/9781071617519en
dc.subjectbioportideen
dc.subjectspermatozoonen
dc.titleProtein mimicry and the design of bioactive cell penetrating peptides: The genesis of STOPSPERM bioportidesen
dc.typeChapter in booken
dc.date.updated2021-09-20T11:31:46Z
dc.identifier.eisbn9781071617526
rioxxterms.funderUniversity of Wolverhamptonen
rioxxterms.identifier.projectUOW21092021JHen
rioxxterms.versionAMen
dc.source.booktitleCell Penetrating Peptides
dc.source.volume2383
dc.source.beginpage293
dc.source.endpage306
refterms.dateFCD2021-09-21T11:32:11Z
refterms.versionFCDAM


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