Actomyosin regulation by eph receptor signaling couples boundary cell formation to border sharpness
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AbstractThe segregation of cells with distinct regional identity underlies formation of a sharp border, which in some tissues serves to organise a boundary signaling centre. It is unclear whether or how border sharpness is coordinated with induction of boundary-specific gene expression. We show that forward signaling of EphA4 is required for border sharpening and induction of boundary cells in the zebrafish hindbrain, which we find both require kinase-dependent signaling, with a lesser input of PDZ domain-dependent signaling. We find that boundary-specific gene expression is regulated by myosin II phosphorylation, which increases actomyosin contraction downstream of EphA4 signaling. Myosin phosphorylation leads to nuclear translocation of Taz, which together with Tead1a is required for boundary marker expression. Since actomyosin contraction maintains sharp borders, there is direct coupling of border sharpness to boundary cell induction that ensures correct organisation of signaling centres.
CitationCayuso, J., Xu, Q., Addison, M. and Wilkinson, D.G. (2019) Actomyosin regulation by Eph receptor signaling couples boundary cell formation to border sharpness. l. eLife 2019;8:e49696. DOI: https://doi.org/10.7554/eLife.4969
PublishereLife Sciences Publications, Ltd
PubMed ID31502954 (pubmed)
Description© 2019 The Authors. Published by eLife Sciences Publications Ltd. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.7554/eLife.49696.001
SponsorsThis work was supported by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001217), the UK Medical Research Council (FC001217), and the Wellcome Trust (FC001217).
Except where otherwise noted, this item's license is described as Licence for published version: Creative Commons Attribution 4.0 International
- Actomyosin regulation by Eph receptor signaling couples boundary cell formation to border sharpness.
- Authors: Cayuso J, Xu Q, Addison M, Wilkinson DG
- Issue date: 2019 Sep 10
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