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dc.contributor.authorWalker, David
dc.contributor.authorVaglio, Stefano
dc.date.accessioned2021-02-15T11:50:06Z
dc.date.available2021-02-15T11:50:06Z
dc.date.issued2021-02-13
dc.identifier.citationWalker, D. and Vaglio, S. (2021) Sampling and analysis of animal scent signals, Journal of Visualized Experiments, 168, e60902. DOI: 10.3791/60902.en
dc.identifier.issn1940-087Xen
dc.identifier.doi10.3791/60902en
dc.identifier.urihttp://hdl.handle.net/2436/623928
dc.descriptionThis is an accepted manuscript of an article published by MyJove Corporation in Journal of Visualized Experiments on 13/02/2021, available online: https://www.jove.com/t/60902/sampling-and-analysis-of-animal-scent-signals?status=a62908k The accepted version of the publication may differ from the final published version.en
dc.description.abstractWe have developed an effective methodology for sampling and analysis of odor signals, by using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry, to understand how they may be used in animal communication. This technique allows the semi-quantitative analysis of the volatile components of odor secretions by enabling the separation and tentative identification of the components in the sample, followed by the analysis of peak area ratios to look for trends that could signify compounds that may be involved in signaling. The key strengths of this current approach are the range of sample types that can be analyzed; the lack of need for any complex sample preparation or extractions; the ability to separate and analyze the components of a mixture; the identification of the components detected; and the capability to provide semi-quantitative and potentially quantitative information on the components detected. The main limitation to the methodology relates to the samples themselves. Since the components of specific interest are volatile, and these could easily be lost, or their concentrations altered, it is important that the samples are stored and transported appropriately after their collection. This also means that sample storage and transport conditions are relatively costly. This method can be applied to a variety of samples (including urine, feces, hair and scent-gland odor secretions). These odors consist of complex mixtures, occurring in a range of matrices, and thus necessitate the use of techniques to separate the individual components and extract the compounds of biological interest.en
dc.formatapplication/pdfen
dc.languageEnglish
dc.language.isoenen
dc.publisherMYJoVE Corporationen
dc.relation.urlhttps://www.jove.com/t/60902/sampling-and-analysis-of-animal-scent-signals?status=a62908ken
dc.subjectheadspace solid-phase microextractionen
dc.subjectgas chromatography-mass spectrometryen
dc.subjectolfactionen
dc.subjectsignallingen
dc.subjectanimal communicationen
dc.subjectscent-markingen
dc.titleSampling and analysis of animal scent signalsen
dc.typeJournal articleen
dc.identifier.journalJournal of Visualized Experimentsen
dc.date.updated2021-02-06T08:54:47Z
dc.identifier.articlenumbere60902
dc.date.accepted2021-01-22
rioxxterms.identifier.project327083en
rioxxterms.identifier.project703611en
rioxxterms.versionAMen
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
rioxxterms.licenseref.startdate2021-08-13en
dc.source.volume168
refterms.dateFCD2021-02-15T11:49:23Z
refterms.versionFCDAM


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