Reduction of shadow band synthesis during PCR amplification of repetitive sequences from modern and ancient DNA
Abstract
Repetitive sequences like short tandem repeat (STR) loci are generally referred to as slippery DNA (1). They owe this nickname to a characteristic leading to slippage within the primer-template complex during PCR elongation of the new strand (2,3), resulting in the synthesis of byproducts shortened by one repeat unit compared with the original sequence. The generation of these so-called shadow bands (4) is a well-known problem connected with the amplification of repetitive DNA, complicating the genotype analysis of modern (e.g., ref. 5), forensic (6), and ancient (7,8) specimens. In some applications, the occurrence of this artifact makes it necessary to develop guidelines for allele designation (6,9).Citation
Schmerer W.M. (2003) Reduction of Shadow Band Synthesis During PCR Amplification of Repetitive Sequences from Modern and Ancient DNA. In: Bartlett J.M.S., Stirling D. (eds) PCR Protocols. Methods in Molecular Biology™, vol 226. Humana Press. https://doi.org/10.1385/1-59259-384-4:309Publisher
Humana PressJournal
Methods in Molecular BiologyPubMed ID
12958513 (pubmed)Additional Links
https://link.springer.com/protocol/10.1385%2F1-59259-384-4%3A309Type
Journal articleLanguage
enDescription
This is an accepted manuscript of an article published by Humana Press in Methods in Molecular Biology in 2013, available online: https://doi.org/10.1385/1-59259-384-4:309 The accepted version of the publication may differ from the final published version.ISSN
1064-3745EISSN
1940-6029ae974a485f413a2113503eed53cd6c53
10.1385/1-59259-384-4:309
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Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by-nc-nd/4.0/
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