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dc.contributor.authorSwale, Andrew
dc.contributor.authorMiyajima, Fabio
dc.contributor.authorKolamunnage-Dona, Ruwanthi
dc.contributor.authorRoberts, Paul
dc.contributor.authorLittle, Margaret
dc.contributor.authorBeeching, Nicholas J
dc.contributor.authorBeadsworth, Mike BJ
dc.contributor.authorLiloglou, Triantafillos
dc.contributor.authorPirmohamed, Munir
dc.date.accessioned2020-08-11T09:30:09Z
dc.date.available2020-08-11T09:30:09Z
dc.date.issued2014-11-15
dc.identifier.citationSwale, A., Miyajima, F., Kolamunnage-Dona, R., Roberts, P. et al. (2014) Serum Mannose-Binding lectin concentration, but not genotype, is associated with Clostridium difficile infection recurrence: a prospective cohort study, Clinical Infectious Diseases, 59(10), pp. 1429-1436en
dc.identifier.issn1058-4838en
dc.identifier.doi10.1093/cid/ciu666en
dc.identifier.urihttp://hdl.handle.net/2436/623465
dc.description© 2014 The Authors. Published by Oxford University Press. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1093/cid/ciu666en
dc.description.abstractLow mannose-binding lectin concentration, but not genotype, was associated with disease recurrence in a large prospective cohort of patients with Clostridium difficile infection. Background. Mannose-binding lectin (MBL) plays a key role in the activation of the lectin-complement pathway of innate immunity, and its deficiency has been linked with several acute infections. However, its role in predisposing to, or modulating disease severity in, Clostridium difficile infection (CDI) has not been investigated. Methods. We prospectively recruited 308 CDI case patients and 145 control patients with antibiotic-associated diarrhea (AAD). CDI outcome measures were disease severity, duration of symptoms, 30-day mortality, and 90-day recurrence. Serum concentrations of MBL were determined using a commercial enzyme-linked immunosorbent assay transferred to an electrochemiluminescence–based platform. MBL2 polymorphisms were typed using a combination of pyrosequencing and TaqMan genotyping assays. Results. The frequency of the MBL2 genetic variants was similar to that reported in other white populations. MBL serum concentrations in CDI and AAD subjects were determined by MBL2 exonic variants B, C, and D and the haplotypes (LYPB, LYQC, and HYPD). There was no difference in either MBL concentrations or genotypes between cases and controls. MBL concentration, but not genotype, was a determinant of CDI recurrence (odds ratios, 3.18 [95% confidence interval {CI}, 1.40–7.24] and 2.61 [95% CI, 1.35–5.04] at the <50 ng/mL and <100 ng/mL cutoff points, respectively; P < .001). However, neither MBL concentration nor MBL2 genotype was linked with the other CDI outcomes. Conclusions. Serum MBL concentration did not differentiate between CDI cases and AAD controls, but among CDI cases, MBL concentration, but not genotype, was associated with CDI recurrence, indicating that MBL acts as a modulator of disease, rather than a predisposing factor.en
dc.description.sponsorshipThis work was funded by the National Institute for Health Research (NIHR) Biomedical Research Centre in microbial diseases in Liverpool, and by the Medical Research Council (MR/K000551/1). We would also like to thank the NIHR for PhD student funding for Andrew Swale (BRF-2011-028). M. P. is an NIHR Senior Investigator.en
dc.formatapplication/pdfen
dc.languageen
dc.language.isoenen
dc.publisherOxford University Press (OUP)en
dc.relation.urlhttps://academic.oup.com/cid/article/59/10/1429/2895675en
dc.subjectclostridium difficileen
dc.subjectCDIen
dc.subjectMBLen
dc.subjectdisease recurrenceen
dc.titleSerum Mannose-Binding lectin concentration, but not genotype, is associated with Clostridium difficile infection recurrence: a prospective cohort studyen
dc.typeJournal articleen
dc.identifier.eissn1537-6591
dc.identifier.journalClinical Infectious Diseasesen
dc.date.updated2020-07-28T11:06:37Z
dc.date.accepted2014-08-09
rioxxterms.funderNIHR, UK MRCen
rioxxterms.identifier.projectMR/K000551/1en
rioxxterms.identifier.projectBRF-2011-028en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2020-08-11en
dc.source.volume59
dc.source.issue10
dc.source.beginpage1429
dc.source.endpage1436
dc.description.versionPublished version
refterms.dateFCD2020-08-11T09:29:09Z
refterms.versionFCDVoR
refterms.dateFOA2020-08-11T00:00:00Z


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