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dc.contributor.authorKumar, Nitin
dc.contributor.authorBrowne, Hilary P.
dc.contributor.authorViciani, Elisa
dc.contributor.authorForster, Samuel C
dc.contributor.authorClare, Simon
dc.contributor.authorHarcourt, Katherine
dc.contributor.authorStares, Mark D
dc.contributor.authorDougan, Gordon
dc.contributor.authorFairley, Derek J
dc.contributor.authorRoberts, Paul
dc.contributor.authorPirmohamed, Munir
dc.contributor.authorClokie, Martha RJ
dc.contributor.authorJensen, Mie Birgitte Frid
dc.contributor.authorHargreaves, Katherine R
dc.contributor.authorIp, Margaret
dc.contributor.authorWieler, Lothar H
dc.contributor.authorSeyboldt, Christian
dc.contributor.authorNoren, Torbjorn
dc.contributor.authorRiley, Thomas V
dc.contributor.authorKuijper, Ed J
dc.contributor.authorWren, Brendan W
dc.contributor.authorLawley, Trevor D
dc.date.accessioned2020-08-07T15:35:17Z
dc.date.available2020-08-07T15:35:17Z
dc.date.issued2019-08-12
dc.identifier.citationKumar, N., Browne, H.P., Viciani, E., Forster, S.C. et al. (2019) Adaptation of host transmission cycle during Clostridium difficile speciation, Nature Genetics 51, pp.1315–1320. https://doi.org/10.1038/s41588-019-0478-8en
dc.identifier.issn1061-4036en
dc.identifier.doi10.1038/s41588-019-0478-8en
dc.identifier.urihttp://hdl.handle.net/2436/623457
dc.descriptionThis is an accepted manuscript of an article published by Nature in Nature Genetics on 12/08/2019, available online: https://doi.org/10.1038/s41588-019-0478-8 The accepted version of the publication may differ from the final published version.en
dc.description.abstractBacterial speciation is a fundamental evolutionary process characterized by diverging genotypic and phenotypic properties. However, the selective forces that affect genetic adaptations and how they relate to the biological changes that underpin the formation of a new bacterial species remain poorly understood. Here, we show that the spore-forming, healthcare-associated enteropathogen Clostridium difficile is actively undergoing speciation. Through large-scale genomic analysis of 906 strains, we demonstrate that the ongoing speciation process is linked to positive selection on core genes in the newly forming species that are involved in sporulation and the metabolism of simple dietary sugars. Functional validation shows that the new C. difficile produces spores that are more resistant and have increased sporulation and host colonization capacity when glucose or fructose is available for metabolism. Thus, we report the formation of an emerging C. difficile species, selected for metabolizing simple dietary sugars and producing high levels of resistant spores, that is adapted for healthcare-mediated transmission.en
dc.formatapplication/pdfen
dc.language.isoenen
dc.publisherNature Researchen
dc.relation.urlhttps://www.nature.com/articles/s41588-019-0478-8en
dc.subjectbacteriologyen
dc.subjectmicrobial geneticsen
dc.subjectmicrobiologyen
dc.titleAdaptation of host transmission cycle during Clostridium difficile speciationen
dc.typeJournal articleen
dc.identifier.journalNature Geneticsen
dc.date.updated2020-07-27T15:50:25Z
dc.date.accepted2019-06-04
rioxxterms.funderMedical Research Councilen
rioxxterms.identifier.projectMR/K000511/1en
rioxxterms.versionAMen
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
rioxxterms.licenseref.startdate2020-08-07en
refterms.dateFCD2020-08-07T15:34:12Z
refterms.versionFCDAM
refterms.dateFOA2020-08-07T00:00:00Z


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