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dc.contributor.authorBernardo, D
dc.contributor.authorMann, ER
dc.contributor.authorAl-Hassi, HO
dc.contributor.authorEnglish, NR
dc.contributor.authorMan, R
dc.contributor.authorLee, GH
dc.contributor.authorRonde, E
dc.contributor.authorLandy, J
dc.contributor.authorPeake, STC
dc.contributor.authorHart, AL
dc.contributor.authorKnight, SC
dc.date.accessioned2020-07-17T15:07:41Z
dc.date.available2020-07-17T15:07:41Z
dc.date.issued2013-09-08
dc.identifier.citationBernardo, D., Mann, E.R., Al-Hassi, H.O. et al (2013) Lost therapeutic potential of monocyte-derived dendritic cells through lost tissue homing: Stable restoration of gut specificity with retinoic acid, Clinical and Experimental Immunology, 174(1), pp. 109-119.en
dc.identifier.issn0009-9104en
dc.identifier.pmid23607934 (pubmed)
dc.identifier.doi10.1111/cei.12118en
dc.identifier.urihttp://hdl.handle.net/2436/623371
dc.description© 2013 The Authors. Published by Wiley. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1111/cei.12118en
dc.description.abstractSummary: Human monocyte-derived dendritic cells (DC) (MoDC) are utilized for immunotherapy. However, in-vitro immunological effects are often not mirrored in vivo. We studied the tissue-homing potential of MoDC. Circulating monocytes and DC expressed different tissue-homing markers and, during in-vitro development of MoDC, homing marker expression was lost resulting in a 'homeless' phenotype. Retinoic acid (RA) induced gut-homing markers (β7 and CCR9) and a regulatory phenotype and function [decreased human leucocyte antigen D-related (HLA-DR) and increased ILT3 and fluorescein isothiocyanate (FITC-dextran uptake) in MoDC]. RA-MoDC were less stimulatory and primed conditioned T cells with a gut-homing profile (β7+CLA-). Unlike the normal intestinal microenvironment, that from inflamed colon of ulcerative colitis (UC) patients did not induce regulatory properties in MoDC. However, RA-MoDC maintained their regulatory gut-specific properties even in the presence of UC microenvironment. Therefore, MoDC may be ineffectual for immunotherapy because they lack tissue-homing and tissue-imprinting specificity. However, MoDC rehabilitation with gut-homing potential by RA could be useful in promoting immunotherapy in pathologies such as UC. © 2013 The Authors. Clinical and Experimental Immunology published by John Wiley & Sons Ltd on behalf of British. Society for Immunology.en
dc.description.sponsorshipThis work was supported by Marie Curie Intra European Fellowship (FP7‐people‐IEF‐2008‐235993), St Mark's Hospital Foundation the Brigid Balfour Fund and the BBSRC (WMNI P33458).en
dc.formatapplication/pdfen
dc.languageeng
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/full/10.1111/cei.12118en
dc.subjectdendritic cellsen
dc.subjecthoming markersen
dc.subjectimmunotherapyen
dc.subjectretinoic aciden
dc.subjectulcerative colitisen
dc.subject.meshGastrointestinal Tract
dc.subject.meshDendritic Cells
dc.subject.meshMonocytes
dc.subject.meshCells, Cultured
dc.subject.meshHumans
dc.subject.meshColitis, Ulcerative
dc.subject.meshTretinoin
dc.subject.meshBiological Markers
dc.subject.meshCell Differentiation
dc.subject.meshCell Movement
dc.subject.meshOrgan Specificity
dc.subject.meshFemale
dc.subject.meshMale
dc.subject.meshReceptors, CCR
dc.subject.meshReceptors, CCR7
dc.titleLost therapeutic potential of monocyte-derived dendritic cells through lost tissue homing: Stable restoration of gut specificity with retinoic aciden
dc.typeJournal articleen
dc.identifier.eissn1365-2249
dc.identifier.journalClinical and Experimental Immunologyen
dc.date.updated2020-06-30T19:30:25Z
dc.contributor.institutionAntigen Presentation Research Group, Imperial College London, Northwick Park & St Mark's Campus, UK.
pubs.place-of-publicationEngland
dc.date.accepted2013-04-05
rioxxterms.funderBiotechnology and Biological Sciences Research Councilen
rioxxterms.identifier.projectBBS/E/F/00044446en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
rioxxterms.licenseref.startdate2020-07-17en
dc.source.volume174
dc.source.issue1
dc.source.beginpage109
dc.source.endpage119
dc.description.versionPublished version
refterms.dateFCD2020-07-17T15:07:30Z
refterms.versionFCDVoR
refterms.dateFOA2020-07-17T15:07:42Z


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