Show simple item record

dc.contributor.authorVora, R
dc.contributor.authorBernardo, D
dc.contributor.authorDurant, L
dc.contributor.authorReddi, D
dc.contributor.authorHart, AL
dc.contributor.authorFell, JME
dc.contributor.authorAl-Hassi, HO
dc.contributor.authorKnight, SC
dc.date.accessioned2020-07-17T14:11:43Z
dc.date.available2020-07-17T14:11:43Z
dc.date.issued2016-03-01
dc.identifier.citationVora, R., Bernardo, D., Durant, L., Reddi, D., Hart, A.L., Fell, J.M.E., Al-Hassi, H.O. and Knight, S.C. (2016) Age-related alterations in blood and colonic dendritic cell properties, Oncotarget, 2016, 7, pp. 11913-11922. https://doi.org/10.18632/oncotarget.7799en
dc.identifier.issn1949-2553en
dc.identifier.pmid26942871 (pubmed)
dc.identifier.doi10.18632/oncotarget.7799en
dc.identifier.urihttp://hdl.handle.net/2436/623367
dc.description© 2016 The Authors. Published by Impact Journals. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.18632/oncotarget.7799en
dc.description.abstractBackground: Dendritic cells (DC) determine initiation, type and location of immune responses and, in adults, show decreased Toll-like receptors and some increased cytokine levels on ageing. Few studies in children have characterised DC or explored DC-related mechanisms producing age-related immune changes. Results: The pDC marker BDCA2 (but not CD123) was absent in pre-pubertal children and numbers of pDC decreased with age. Blood and colonic DC were more mature and activated in adults. Decrease in pDC numbers correlated with reduced GM-CSF levels with aging, but increasing IL-4 and IL-8 levels correlated with a more activated DC profile in blood. CXCL16 levels decreased with age. Methods: Blood and colonic DC phenotypes were determined in healthy adults and children by flow cytometry and correlated with aging. Blood DC were divided into plasmacytoid (pDC) and myeloid (mDC) while only mDC were identified in colon. Serum cytokine levels were determined by multiplex cytokine assays and correlated with DC properties. Conclusions: In children, lack of BDCA2, a receptor mediating antigen capture and inhibiting interferon induction, may be immunologically beneficial during immune development. Conversely, reduced pDC numbers, probably secondary to decreasing GM-CSF and increasing cytokine-induced maturation of DC are likely to determine deteriorating immunity with ageing.en
dc.description.sponsorshipThe authors gratefully acknowledge the support of the Biotechnology and Biological Sciences Research Council (BBSRC). This research was funded by the BBSRC Institute Strategic Programme for Gut Health and Food Safety BB/J004529/1. The authors also gratefully acknowledge the support of the Westminster Medical School Research Trust and Crohn’s in Childhood Research Association (Reference JRC SG 002 03/13-14)en
dc.formatapplication/pdfen
dc.languageeng
dc.language.isoenen
dc.publisherImpact Journals, LLCen
dc.relation.urlhttps://www.oncotarget.com/article/7799/text/en
dc.rightsLicence for published version: Creative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectmDCen
dc.subjectpDCen
dc.subjectchildrenen
dc.subjectagingen
dc.subjectcytokinesen
dc.subjectBDCA-2en
dc.subjectgerotargeten
dc.subject.meshColon
dc.subject.meshDendritic Cells
dc.subject.meshCells, Cultured
dc.subject.meshMyeloid Cells
dc.subject.meshHumans
dc.subject.meshCytokines
dc.subject.meshAge Factors
dc.subject.meshCell Differentiation
dc.subject.meshAdult
dc.subject.meshChild
dc.subject.meshBiomarkers
dc.titleAge-related alterations in blood and colonic dendritic cell propertiesen
dc.typeJournal articleen
dc.identifier.eissn1949-2553
dc.identifier.journalOncotargeten
dc.date.updated2020-06-30T19:26:35Z
dc.contributor.institutionChelsea and Westminster Hospital NHS Foundation Trust, London, UK.
pubs.place-of-publicationUnited States
dc.date.accepted2016-01-30
rioxxterms.funderBiotechnology and Biological Sciences Research Councilen
rioxxterms.identifier.projectBBS/E/F/00044446en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2020-07-17en
dc.source.volume7
dc.source.issue11
dc.source.beginpage11913
dc.source.endpage11922
dc.description.versionPublished version
refterms.dateFCD2020-07-17T14:09:41Z
refterms.versionFCDVoR
refterms.dateFOA2020-07-17T14:11:44Z


Files in this item

Thumbnail
Name:
Age-related alterations in blood ...
Size:
2.550Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

Licence for published version: Creative Commons Attribution 4.0 International
Except where otherwise noted, this item's license is described as Licence for published version: Creative Commons Attribution 4.0 International