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dc.contributor.authorLandy, J
dc.contributor.authorWalker, AW
dc.contributor.authorLi, JV
dc.contributor.authorAl-Hassi, HO
dc.contributor.authorRonde, E
dc.contributor.authorEnglish, NR
dc.contributor.authorMann, ER
dc.contributor.authorBernardo, D
dc.contributor.authorMcLaughlin, SD
dc.contributor.authorParkhill, J
dc.contributor.authorCiclitira, PJ
dc.contributor.authorClark, SK
dc.contributor.authorKnight, SC
dc.contributor.authorHart, AL
dc.date.accessioned2020-07-17T13:58:26Z
dc.date.available2020-07-17T13:58:26Z
dc.date.issued2015-08-12
dc.identifier.citationLandy, J., Walker, A., Li, J. et al. (2015) Variable alterations of the microbiota, without metabolic or immunological change, following faecal microbiota transplantation in patients with chronic pouchitis. Scientific Reports, 5, 12955. https://doi.org/10.1038/srep12955en
dc.identifier.issn2045-2322en
dc.identifier.pmid26264409 (pubmed)
dc.identifier.doi10.1038/srep12955en
dc.identifier.urihttp://hdl.handle.net/2436/623366
dc.description© 2015 The Authors. Published by Springer Nature. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1038/srep12955en
dc.description.abstractFaecal microbiota transplantation (FMT) is effective in the treatment of Clostridium difficile infection, where efficacy correlates with changes in microbiota diversity and composition. The effects of FMT on recipient microbiota in inflammatory bowel diseases (IBD) remain unclear. We assessed the effects of FMT on microbiota composition and function, mucosal immune response, and clinical outcome in patients with chronic pouchitis. Eight patients with chronic pouchitis (current PDAI ‰7) were treated with FMT via nasogastric administration. Clinical activity was assessed before and four weeks following FMT. Faecal coliform antibiotic sensitivities were analysed, and changes in pouch faecal and mucosal microbiota assessed by 16S rRNA gene pyrosequencing and 1 H NMR spectroscopy. Lamina propria dendritic cell phenotype and cytokine profiles were assessed by flow cytometric analysis and multiplex assay. Following FMT, there were variable shifts in faecal and mucosal microbiota composition and, in some patients, changes in proportional abundance of species suggestive of a 'healthier' pouch microbiota. However, there were no significant FMT-induced metabolic or immunological changes, or beneficial clinical response. Given the lack of clinical response following FMT via a single nasogastric administration our results suggest that FMT/bacteriotherapy for pouchitis patients requires further optimisation.en
dc.formatapplication/pdfen
dc.languageeng
dc.language.isoenen
dc.publisherSpringer Science and Business Media LLCen
dc.relation.urlhttps://www.nature.com/articles/srep12955en
dc.rightsLicence for published version: Creative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectbacterial host responseen
dc.subjecttranslational researchen
dc.subject.meshHumans
dc.subject.meshPouchitis
dc.subject.meshChronic Disease
dc.subject.meshAdult
dc.subject.meshMiddle Aged
dc.subject.meshFemale
dc.subject.meshMale
dc.subject.meshImmunity, Innate
dc.subject.meshMetabolomics
dc.subject.meshProton Magnetic Resonance Spectroscopy
dc.subject.meshFecal Microbiota Transplantation
dc.titleVariable alterations of the microbiota, without metabolic or immunological change, following faecal microbiota transplantation in patients with chronic pouchitisen
dc.typeJournal articleen
dc.identifier.eissn2045-2322
dc.identifier.journalScientific Reportsen
dc.date.updated2020-06-30T19:25:03Z
dc.contributor.institution1] IBD Unit, Gastroenterology Dept. St Mark's Hospital, Harrow, London, UK [2] Antigen Presentation Research Group, Faculty of Medicine, Imperial College London, Northwick Park and St Mark's Campus, Harrow, UK.
dc.identifier.articlenumber12955
pubs.place-of-publicationEngland
dc.date.accepted2015-07-06
rioxxterms.funderImperial College Londonen
rioxxterms.identifier.projectWMNIP33458en
rioxxterms.identifier.projectWT098051en
rioxxterms.identifier.projectBBS/E/F/00044446en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2020-07-17en
dc.source.volume5
dc.source.issue1
dc.description.versionPublished version
refterms.dateFCD2020-07-17T13:58:12Z
refterms.versionFCDVoR
refterms.dateFOA2020-07-17T13:58:27Z


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Licence for published version: Creative Commons Attribution 4.0 International
Except where otherwise noted, this item's license is described as Licence for published version: Creative Commons Attribution 4.0 International