Variable alterations of the microbiota, without metabolic or immunological change, following faecal microbiota transplantation in patients with chronic pouchitis
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Authors
Landy, JWalker, AW
Li, JV
Al-Hassi, HO
Ronde, E
English, NR
Mann, ER
Bernardo, D
McLaughlin, SD
Parkhill, J
Ciclitira, PJ
Clark, SK
Knight, SC
Hart, AL
Issue Date
2015-08-12
Metadata
Show full item recordAbstract
Faecal microbiota transplantation (FMT) is effective in the treatment of Clostridium difficile infection, where efficacy correlates with changes in microbiota diversity and composition. The effects of FMT on recipient microbiota in inflammatory bowel diseases (IBD) remain unclear. We assessed the effects of FMT on microbiota composition and function, mucosal immune response, and clinical outcome in patients with chronic pouchitis. Eight patients with chronic pouchitis (current PDAI ‰7) were treated with FMT via nasogastric administration. Clinical activity was assessed before and four weeks following FMT. Faecal coliform antibiotic sensitivities were analysed, and changes in pouch faecal and mucosal microbiota assessed by 16S rRNA gene pyrosequencing and 1 H NMR spectroscopy. Lamina propria dendritic cell phenotype and cytokine profiles were assessed by flow cytometric analysis and multiplex assay. Following FMT, there were variable shifts in faecal and mucosal microbiota composition and, in some patients, changes in proportional abundance of species suggestive of a 'healthier' pouch microbiota. However, there were no significant FMT-induced metabolic or immunological changes, or beneficial clinical response. Given the lack of clinical response following FMT via a single nasogastric administration our results suggest that FMT/bacteriotherapy for pouchitis patients requires further optimisation.Citation
Landy, J., Walker, A., Li, J. et al. (2015) Variable alterations of the microbiota, without metabolic or immunological change, following faecal microbiota transplantation in patients with chronic pouchitis. Scientific Reports, 5, 12955. https://doi.org/10.1038/srep12955Publisher
Springer Science and Business Media LLCJournal
Scientific ReportsPubMed ID
26264409 (pubmed)Additional Links
https://www.nature.com/articles/srep12955Type
Journal articleLanguage
enDescription
© 2015 The Authors. Published by Springer Nature. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1038/srep12955ISSN
2045-2322EISSN
2045-2322ae974a485f413a2113503eed53cd6c53
10.1038/srep12955
Scopus Count
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Except where otherwise noted, this item's license is described as Licence for published version: Creative Commons Attribution 4.0 International
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