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dc.contributor.authorMann, ER
dc.contributor.authorYou, J
dc.contributor.authorHorneffer-Van Der Sluis, V
dc.contributor.authorBernardo, D
dc.contributor.authorOmar Al-Hassi, H
dc.contributor.authorLandy, J
dc.contributor.authorPeake, ST
dc.contributor.authorThomas, LV
dc.contributor.authorTee, CT
dc.contributor.authorLee, GH
dc.contributor.authorHart, AL
dc.contributor.authorYaqoob, P
dc.contributor.authorKnight, SC
dc.date.accessioned2020-07-17T08:38:41Z
dc.date.available2020-07-17T08:38:41Z
dc.date.issued2013-07-18
dc.identifier.citationMann, E. R., You, J., Horneffer-van der Sluis, V., Bernardo, D., Omar Al-Hassi, H., Landy, J., Peake, S. T., Thomas, L. V., Tee, C. T., Lee, G. H., Hart, A. L., Yaqoob, P. and Knight, S. C. (2013) Dysregulated circulating dendritic cell function in ulcerative colitis is partially restored by probiotic strain Lactobacillus casei Shirota. Mediators of inflammation, 2013. 573576.en
dc.identifier.issn0962-9351en
dc.identifier.pmid23970814 (pubmed)
dc.identifier.doi10.1155/2013/573576en
dc.identifier.urihttp://hdl.handle.net/2436/623363
dc.description© 2013 The Authors. Published by Hindawi. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1155/2013/573576en
dc.description.abstractBackground. Dendritic cells regulate immune responses to microbial products and play a key role in ulcerative colitis (UC) pathology. We determined the immunomodulatory effects of probiotic strain Lactobacillus casei Shirota (LcS) on human DC from healthy controls and active UC patients. Methods. Human blood DC from healthy controls (control-DC) and UC patients (UC-DC) were conditioned with heat-killed LcS and used to stimulate allogeneic T cells in a 5-day mixed leucocyte reaction. Results. UC-DC displayed a reduced stimulatory capacity for T cells (P<0.05) and enhanced expression of skin-homing markers CLA and CCR4 on stimulated T cells (P<0.05) that were negative for gut-homing marker β7. LcS treatment restored the stimulatory capacity of UC-DC, reflecting that of control-DC. LcS treatment conditioned control-DC to induce CLA on T cells in conjunction with β7, generating a multihoming profile, but had no effects on UC-DC. Finally, LcS treatment enhanced DC ability to induce TGFβ production by T cells in controls but not UC patients. Conclusions. We demonstrate a systemic, dysregulated DC function in UC that may account for the propensity of UC patients to develop cutaneous manifestations. LcS has multifunctional immunoregulatory activities depending on the inflammatory state; therapeutic effects reported in UC may be due to promotion of homeostasis. © 2013 Elizabeth R. Mann et al.en
dc.description.sponsorshipThis research was funded by Yakult Europe B.V. (Almere, The Netherlands) and the L. casei Shirota was provided by Yakult Honsha Co. Ltd. (Tokyo, Japan). The paper was supported by a grant from Yakult Europe B.V., The Netherlands.en
dc.formatapplication/pdfen
dc.languageeng
dc.language.isoenen
dc.publisherHindawi Limiteden
dc.relation.urlhttps://www.hindawi.com/journals/mi/2013/573576/en
dc.rightsLicence for published version: Creative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshDendritic Cells
dc.subject.meshT-Lymphocytes
dc.subject.meshCells, Cultured
dc.subject.meshHumans
dc.subject.meshLactobacillus casei
dc.subject.meshColitis, Ulcerative
dc.subject.meshInflammation
dc.subject.meshTransforming Growth Factor beta
dc.subject.meshCytokines
dc.subject.meshFlow Cytometry
dc.subject.meshLymphocyte Activation
dc.subject.meshCell Proliferation
dc.subject.meshHomeostasis
dc.subject.meshProbiotics
dc.titleDysregulated circulating dendritic cell function in ulcerative colitis is partially restored by probiotic strain lactobacillus casei shirotaen
dc.typeJournal articleen
dc.identifier.eissn1466-1861
dc.identifier.journalMediators of Inflammationen
dc.date.updated2020-06-30T19:19:14Z
dc.contributor.institutionAntigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark's Campus, Level 7W St. Mark's Hospital, Watford Road, Harrow HA1 3UJ, UK.
pubs.place-of-publicationUnited States
dc.date.accepted2013-05-30
rioxxterms.funderBiotechnology and Biological Sciences Research Councilen
rioxxterms.identifier.projectBBS/E/F/00044446en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2020-07-17en
dc.source.volume2013
dc.source.beginpage573576
dc.description.versionPublished version
refterms.dateFCD2020-07-17T08:38:26Z
refterms.versionFCDVoR
refterms.dateFOA2020-07-17T08:38:42Z


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Licence for published version: Creative Commons Attribution 4.0 International
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