Dysregulated circulating dendritic cell function in ulcerative colitis is partially restored by probiotic strain lactobacillus casei shirota
Authors
Mann, ERYou, J
Horneffer-Van Der Sluis, V
Bernardo, D
Omar Al-Hassi, H
Landy, J
Peake, ST
Thomas, LV
Tee, CT
Lee, GH
Hart, AL
Yaqoob, P
Knight, SC
Issue Date
2013-07-18
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Background. Dendritic cells regulate immune responses to microbial products and play a key role in ulcerative colitis (UC) pathology. We determined the immunomodulatory effects of probiotic strain Lactobacillus casei Shirota (LcS) on human DC from healthy controls and active UC patients. Methods. Human blood DC from healthy controls (control-DC) and UC patients (UC-DC) were conditioned with heat-killed LcS and used to stimulate allogeneic T cells in a 5-day mixed leucocyte reaction. Results. UC-DC displayed a reduced stimulatory capacity for T cells (P<0.05) and enhanced expression of skin-homing markers CLA and CCR4 on stimulated T cells (P<0.05) that were negative for gut-homing marker β7. LcS treatment restored the stimulatory capacity of UC-DC, reflecting that of control-DC. LcS treatment conditioned control-DC to induce CLA on T cells in conjunction with β7, generating a multihoming profile, but had no effects on UC-DC. Finally, LcS treatment enhanced DC ability to induce TGFβ production by T cells in controls but not UC patients. Conclusions. We demonstrate a systemic, dysregulated DC function in UC that may account for the propensity of UC patients to develop cutaneous manifestations. LcS has multifunctional immunoregulatory activities depending on the inflammatory state; therapeutic effects reported in UC may be due to promotion of homeostasis. © 2013 Elizabeth R. Mann et al.Citation
Mann, E. R., You, J., Horneffer-van der Sluis, V., Bernardo, D., Omar Al-Hassi, H., Landy, J., Peake, S. T., Thomas, L. V., Tee, C. T., Lee, G. H., Hart, A. L., Yaqoob, P. and Knight, S. C. (2013) Dysregulated circulating dendritic cell function in ulcerative colitis is partially restored by probiotic strain Lactobacillus casei Shirota. Mediators of inflammation, 2013. 573576.Publisher
HindawiJournal
Mediators of InflammationPubMed ID
23970814 (pubmed)Additional Links
https://www.hindawi.com/journals/mi/2013/573576/Type
Journal articleLanguage
enDescription
© 2013 The Authors. Published by Hindawi. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1155/2013/573576ISSN
0962-9351EISSN
1466-1861Sponsors
This research was funded by Yakult Europe B.V. (Almere, The Netherlands) and the L. casei Shirota was provided by Yakult Honsha Co. Ltd. (Tokyo, Japan). The paper was supported by a grant from Yakult Europe B.V., The Netherlands.ae974a485f413a2113503eed53cd6c53
10.1155/2013/573576
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