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dc.contributor.authorRomero-Lorca, A
dc.contributor.authorGaibar, M
dc.contributor.authorArmesilla, AL
dc.contributor.authorFernandez-Santander, ANA
dc.contributor.authorNovillo, A
dc.date.accessioned2020-03-04T14:39:58Z
dc.date.available2020-03-04T14:39:58Z
dc.date.issued2018-10-02
dc.identifier.citationRomero-Lorca, A., Gaibar, M., Armesilla, A. L., Fernandez-Santander, A. and Novillo, A. (2018) Differential expression of PMCA2 mRNA isoforms in a cohort of Spanish patients with breast tumor types, Oncology Letters, 16(6), pp. 6950-6959.en
dc.identifier.issn1792-1074en
dc.identifier.doi10.3892/ol.2018.9540en
dc.identifier.urihttp://hdl.handle.net/2436/623117
dc.description© 2018 The Authors. Published by Spandidos Publications. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.3892/ol.2018.9540en
dc.description.abstract© Romero-Lorca et al. The present study examined the mRNA expression levels of different isoforms of the plasma membrane calcium ATPase 2 (PMCA2) gene generated by alternative splicing at the first intracellular loop (site A) and C-terminal region (site C) in 85 human breast cancer tumor and 69 adjacent non-tumor tissues. Associations were identified between the expression of PMCA2 splice isoforms and the following clinical variables: Estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) status, tumor size, staging and histological classification, and lymph node status. Transcripts including splice site A or splice site C were amplified by reverse transcription-quantitative polymerase chain reaction using PMCA2 isoform-specific primers. Tumor and adjacent tissues were determined to express the different PMCA2 splice isoforms 2w, 2x and 2z (site A), and 2b (site C). The mRNA levels for these variants indicated high biological variability, but increased expression was observed in breast tumor tissues, compared with in adjacent tissues. Significantly increased PMCA2x/b expression levels were detected in breast tumor tissues histologically classified as lobulillar, compared with in ductal-types breast tumor tissues (P<0.028). Furthermore, PMCA2z expression was significantly associated with PR status (P<0.024, compared with in PR-negative tumor tissues), and PMCA2w expression was significantly associated with ER status (P<0.048, increased in ER-positive tumor tissues, compared with ER-negative tumor tissues). Finally, PMCA2b was overexpressed in HER2-positive tumor tissues, compared with in HER2-negative tumor tissues (P<0.014). The data demonstrated the differential mRNA expression of a number of splice site A and C variants of PMCA2 in breast tumor and adjacent tissues, depending on tumor hormone receptor status and histological classification. In agreement with previous data, PMCA2b was overexpressed in HER2-positive tumor tissues, indicating that high mRNA levels of this variant could be a marker of poor prognosis.en
dc.description.sponsorshipThe present study was funded by the Universidad Europea de Madrid (project 2014/UEM005).en
dc.formatapplication/pdfen
dc.language.isoenen
dc.publisherSpandidos Publicationsen
dc.relation.urlhttps://www.spandidos-publications.com/10.3892/ol.2018.9540en
dc.subjectPMCA2en
dc.subjectalternative splicingen
dc.subjectbreast canceren
dc.titleDifferential expression of PMCA2 mRNA isoforms in a cohort of Spanish patients with breast tumor typesen
dc.typeJournal articleen
dc.identifier.eissn1792-1082
dc.identifier.journalOncology Lettersen
dc.date.updated2020-03-03T09:12:26Z
dc.date.accepted2018-05-29
rioxxterms.funderWellcome Trusten
rioxxterms.identifier.project2014/UEM005en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
rioxxterms.licenseref.startdate2020-03-04en
dc.source.volume16
dc.source.issue6
dc.source.beginpage6950
dc.source.endpage6959
dc.description.versionPublished version
refterms.dateFCD2020-03-04T14:38:17Z
refterms.versionFCDVoR
refterms.dateFOA2020-03-04T14:39:58Z


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