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dc.contributor.authorHeaselgrave, Wayne
dc.contributor.authorHamad, Anas
dc.contributor.authorColes, Steven
dc.contributor.authorHau, Scott
dc.date.accessioned2019-10-02T15:34:51Z
dc.date.available2019-10-02T15:34:51Z
dc.date.issued2019-09-25
dc.identifier.citationHeaselgrave W, Hamad A, Coles S, Hau S. In vitro evaluation of the inhibitory effect of topical ophthalmic agents on Acanthamoeba viability. Trans Vis Sci Tech. 2019; 8(5):17, https://doi.org/10.1167/ tvst.8.5.17en
dc.identifier.issn2164-2591en
dc.identifier.doi10.1167/tvst.8.5.17en
dc.identifier.urihttp://hdl.handle.net/2436/622763
dc.description.abstractPurpose: To compare the antimicrobial effect of topical anesthetics, antivirals, antibiotics, and biocides on the viability of Acanthamoeba cysts and trophozoites in vitro. Methods: Amoebicidal and cysticidal assays were performed against both trophozoites and cysts of Acanthamoeba castellanii (ATCC 50370) and Acanthamoeba polyphaga (ATCC 30461). Test agents included topical ophthalmic preparations of common anesthetics, antivirals, antibiotics, and biocides. Organisms were exposed to serial two-fold dilutions of the test compounds in the wells of a microtiter plate to examine the effect on Acanthamoeba spp. In addition, the toxicity of each of the test compounds was determined against a mammalian cell line. Results: Proxymetacaine, oxybuprocaine, and especially tetracaine were all toxic to the trophozoites and cysts of Acanthamoeba spp., but lidocaine was well tolerated. The presence of the benzalkonium chloride (BAC) preservative in levofloxacin caused a high level of toxicity to trophozoites and cysts. With the diamidines, the presence of BAC in the propamidine drops was responsible for the activity against Acanthamoeba spp. Hexamidine drops without BAC showed good activity against trophozoites, and the biguanides polyhexamethylene biguanide, chlorhexidine, alexidine, and octenidine all showed excellent activity against trophozoites and cysts of both species. Conclusions: The antiamoebic effects of BAC, povidone iodine, and tetracaine are superior to the current diamidines and slightly inferior to the biguanides used in the treatment for Acanthamoeba keratitis. Translational Relevance: Ophthalmologists should be aware that certain topical anesthetics and ophthalmic preparations containing BAC prior to specimen sampling may affect the viability of Acanthamoeba spp. in vivo, resulting in false-negative results in diagnostic tests.en
dc.formatapplication/PDFen
dc.languageEnglish
dc.language.isoenen
dc.publisherARVO Journalsen
dc.relation.urlhttps://tvst.arvojournals.org/article.aspx?articleid=2752036&resultClick=1en
dc.subjectKeratitisen
dc.subjectAcanthamoeba sppen
dc.subjectTreatmenten
dc.titleIn vitro evaluation of the inhibitory effect of topical ophthalmic agents on acanthamoeba viabilityen
dc.typeJournal articleen
dc.identifier.journalTranslational Vision Science and Technologyen
dc.date.updated2019-09-25T09:21:59Z
dc.identifier.articlenumber17
dc.date.accepted2019-07-28
rioxxterms.funderJiscen
rioxxterms.identifier.projectUOW02102019WHen
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2019-10-02en
dc.source.volume8
dc.source.issue5
refterms.dateFCD2019-10-02T15:34:17Z
refterms.versionFCDVoR
refterms.dateFOA2019-10-02T15:34:52Z


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