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dc.contributor.authorPoulianiti, KP
dc.contributor.authorKarioti, A
dc.contributor.authorKaltsatou, A
dc.contributor.authorMitrou, GI
dc.contributor.authorKoutedakis, Y
dc.contributor.authorTepetes, K
dc.contributor.authorChristodoulidis, G
dc.contributor.authorGiakas, G
dc.contributor.authorMaridaki, MD
dc.contributor.authorStefanidis, I
dc.contributor.authorJamurtas, AZ
dc.contributor.authorSakkas, GK
dc.contributor.authorKaratzaferi, C
dc.date.accessioned2019-08-21T10:40:55Z
dc.date.available2019-08-21T10:40:55Z
dc.date.issued2019-04-04
dc.identifier.citationPoulianiti, K. et al. (2019) Evidence of blood and muscle redox status imbalance in experimentally induced renal insufficiency in a rabbit model, Oxidative Medicine and Cellular Longevity (2019), DOI: 10.1155/2019/8219283.en
dc.identifier.issn1942-0900en
dc.identifier.pmid31089418
dc.identifier.doi10.1155/2019/8219283en
dc.identifier.urihttp://hdl.handle.net/2436/622669
dc.description.abstractChronic kidney disease (CKD) is accompanied by a disturbed redox homeostasis, especially in end-stage patients, which is associated with pathological complications such as anemia, atherosclerosis, and muscle atrophy. However, limited evidence exists about redox disturbances before the end stage of CKD. Moreover, the available redox literature has not yet provided clear associations between circulating and tissue-specific (muscle) oxidative stress levels. The aim of the study was to evaluate commonly used redox status indices in the blood and in two different types of skeletal muscle (psoas, soleus) in the predialysis stages of CKD, using an animal model of renal insufficiency, and to investigate whether blood redox status indices could be reflecting the skeletal muscle redox status. Indices evaluated included reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione reductase (GR), catalase (CAT), total antioxidant capacity (TAC), protein carbonyls (PC), and thiobarbituric acid reactive substances (TBARS). Results showed that blood GSH was higher in the uremic group compared to the control (17.50 ± 1.73 vs. 12.43 ± 1.01, p = 0.033). In both muscle types, PC levels were higher in the uremic group compared to the control (psoas: 1.086 ± 0.294 vs. 0.596 ± 0.372, soleus: 2.52 ± 0.29 vs. 0.929 ± 0.41, p < 0.05). The soleus had higher levels of TBARS, PC, GSH, CAT, and GR and lower TAC compared to the psoas in both groups. No significant correlations in redox status indices between the blood and skeletal muscles were found. However, in the uremic group, significant correlations between the psoas and soleus muscles in PC, GSSG, and CAT levels emerged, not present in the control. Even in the early stages of CKD, a disturbance in redox homeostasis was observed, which seemed to be muscle type-specific, while blood levels of redox indices did not seem to reflect the intramuscular condition. The above results highlight the need for further research in order to identify the key mechanisms driving the onset and progression of oxidative stress and its detrimental effects on CKD patients.en
dc.description.sponsorshipThis work was supported by the European Union (European Social Fund, ESF) and Greek national funds through the Operational Program “Educational and Lifelong Learning” of the National Strategic Reference Framework (NSRF), Research Funding Program: Thales (MuscleFun Project-MIS 377260) Investing in Knowledge Society through the European Social Fund, and the European Union Horizon 2020 Research and Innovation Programme “H2020 MSCAS-RISE-Muscle Stress Relief” under grant agreement no. 645648.en
dc.formatapplication/PDFen
dc.languageeng
dc.language.isoenen
dc.publisherHindawi Limiteden
dc.relation.urlhttps://www.hindawi.com/journals/omcl/2019/8219283/en
dc.rightsLicence for published version: Creative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleEvidence of blood and muscle redox status imbalance in experimentally induced renal insufficiency in a rabbit modelen
dc.typeJournal articleen
dc.identifier.eissn1942-0994
dc.identifier.journalOxidative Medicine and Cellular Longevityen
dc.date.updated2019-08-20T13:22:14Z
dc.contributor.institutionMuscle Physiology & Mechanics Group, CREHP, DPESS, University of Thessaly, Trikala 42100, Greece.
pubs.place-of-publicationUnited States
dc.date.accepted2019-02-28
rioxxterms.funderUniversity of Wolverhamptonen
rioxxterms.identifier.projectMIS 377260en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2019-08-21en
dc.source.volume2019
dc.source.beginpage8219283
dc.description.versionPublished version
refterms.dateFCD2019-08-21T10:40:41Z
refterms.versionFCDVoR
refterms.dateFOA2019-08-21T10:40:55Z


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Licence for published version: Creative Commons Attribution 4.0 International
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